Toluidine Blue O Stain Solution (For Cells) - BioReagent,Biological Stain,for microscopy

Cat. No.: T1511051

GRADE & PURITY BioReagent, Biological Stain, for Microscopy

Synonyms

Toluidine Blue O Staining Solution | Toluidine Blue Staining Solution | Mast Cell Staining Solution

Storage

Store at 2-8°C,Protected from light

Shipped In

Wet ice

Application

Cell Staining

Overview

  The cations in Toluidine Blue O exert the staining effect, and acidic substances in tissue cells combine with these cations to be stained. Toluidine Blue O also contains two auxochromes that promote the ionization of the dye to form salts, enhance the staining power of chromophores on tissues, and thus stain the tissue cells on sections-specifically, it can stain cell nuclei blue. Clinically, Toluidine Blue O is frequently used to stain chondrocytes and mast cells.

  Toluidine Blue O Stain Solution (For Cells) is suitable for staining acidic mucopolysaccharides in cells, especially for staining sulfur-containing acidic mucous substances, which appear red after staining.

Operating Procedures (For Reference Only):

1. After preparing the cell smear, add Toluidine Blue Staining Solution (Cell-Specific) dropwise and stain for 5 minutes.

2. Add an equal volume of distilled water to the smear, mix well, and stain for another 15 minutes.

3. Rinse with water, air-dry the smear, and examine under a microscope.

Staining Result:

  Acidic mucopolysaccharides in cells appear red.

Precautions: 

1. To verify the staining effect, blood smears from chronic myeloid leukemia (eosinophils) or bone marrow smears from aplastic anemia (tissue eosinophils) can be used as controls.

2. For your safety and health, wear a lab coat and disposable gloves during operation.

3. This product is for research use only and shall not be used for any other purposes.

Specifications

Synonyms

Toluidine Blue O Staining Solution | Toluidine Blue Staining Solution | Mast Cell Staining Solution

Specifications & Purity

BioReagent, Biological Stain, for microscopy

Stability And Storage

Store at 2-8℃ long term (12 months). Store in the dark.

Storage

Store at 2-8°C, Protected from light

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Biological Stain, BioReagent, for Microscopy

Chemical and Physical Properties

• Sensitivity: Light-sensitive