Trypsin Inhibitor from Soybean, CAS No.9035-81-8

CAS: 9035-81-8 Cat. No.: S752084 EC Number: 232-906-9

GRADE & PURITY ActiBioPure™, Native, for Cell culture, ≥0.7mg Trypsin/mg Inhibitor (Trypsin activity is about 10000 BAEE units/mg)

Accession #

P01070

Bioactivity

≥0.7mg Trypsin/mg Inhibitor (Trypsin activity is about 10000 BAEE units/mg)

Overview

  This product is a natural active protein extracted from non-genetically modified soybeans, containing only the Kunitz Trypsin Inhibitor (KTI) protein component. It has trypsin inhibitor activity but no chymotrypsin (also known as chymase) inhibitor activity. The trypsin inhibitor (Trypsin Inhibitor, TI) activity of this product is ≥10000 BAEE U/mg, and it has high purity, making it suitable for experiments such as biochemical detection and cell culture.

Soybean protease inhibitors include two types of trypsin inhibitors: Bowman-Birk Inhibitor (BBI) and Kunitz Trypsin Inhibitor (KTI). BBI is a protein with a molecular weight of about 8KD Daltons, which has the property of inhibiting trypsin and chymotrypsin; KTI is a protein with a molecular weight of about 23KD Daltons, which has the property of inhibiting trypsin and is a stable globulin-type protein.

Product Characteristics

Item	Acceptance Criteria
Appearance	White or off-white powder
Clarity and Color	Clear, colorless to pale yellow solution
Moisture	Exceeding 10%
Trypsin Inhibitor Activity	Not less than 0.7mg trypsin/mg (trypsin activity is about 10000 BAEE units/mg)

Product Advantages

  This product is extracted from non-genetically modified soybeans, with high purity, no other miscellaneous proteases, and no animal-derived safety risks throughout the entire process.

Product Uses

  Soybean trypsin inhibitor has trypsin inhibitor and chymotrypsin inhibitor activities, and is commonly used in serum-free cell culture, stem cell culture, pharmaceutical excipients, or other biochemical experiments that require protease inhibition.

Specifications

Product Name

Trypsin Inhibitor from Soybean, CAS No.9035-81-8

Synonyms

SBTI | Kunitz Trypsin Inhibitor | KTI | KTI3

Grade

ActiBioPure™, for Cell culture, Native

Specifications & Purity

ActiBioPure™, Native, for cell culture, ≥0.7mg Trypsin/mg Inhibitor (Trypsin activity is about 10000 BAEE units/mg)

Biochemical and Physiological Mechanisms

Inhibition of trypsin. The sequence of variant A is shown. Electrophoresis identifies three genetically distinct variants, A, B, and C, that are inherited as codominant alleles.

Bioactivity

≥0.7mg Trypsin/mg Inhibitor (Trypsin activity is about 10000 BAEE units/mg)

Accession #

P01070

Predicted molecular weight

23 kDa

CAS

9035-81-8

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥0.7mg Trypsin/mg Inhibitor (Trypsin activity is about 10000 BAEE units/mg)

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (24 months). Upon reconstitution, it is recommended to aliquot.

References

1. Fanping Shi, Lei Wang, Yan Li, Yu Zhang, Xingguang Su.  (2017)  A simple “turn-on” detection platform for trypsin activity and inhibitor screening based on N-acetyl-l-cysteine capped CdTe Quantum Dots.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2017.09.087]

2. Wei-Xian Wang, Yuqing Wu, Hong-Wei Li.  (2017)  Regulation on the aggregation-induced emission (AIE) of DNA-templated silver nanoclusters by BSA and its hydrolysates.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:28646761] [10.1016/j.jcis.2017.06.033]

3. Xiu Huang, Yong Li, Zhao Shu, Li Huang, Qian Liu, Guibin Jiang.  (2024)  High-Efficiency Degradation of PET Plastics by Glutathione S-Transferase under Mild Conditions.  ENVIRONMENTAL SCIENCE & TECHNOLOGY,      [PMID:39012182] [10.1021/acs.est.4c02132]

4. Fan Yanan, Xu Qixiang, Ren Keyu, Zhai Mengge, Xing Guozheng, Song Yishan, Zhu Yongheng.  (2024)  Research on pH-responsive Antibacterial Materials Using Citral-modified Zinc Oxide Nanoparticles.  Food Quality and Safety,      [PMID:] [10.1093/fqsafe/fyae010]

5. Xiu Huang, Li Huang, Qian Wang, Qiurong He, Zunzhen Zhang, Qian Liu, Guibin Jiang.  (2025)  High-efficiency enzymatic biodegradation of polypropylene-based melt-blown fabric debris.  Chemical Science,      [PMID:40708876] [10.1039/D5SC03097H]