Tubulin-Tracker Green

Cat. No.: T751440

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.Coverslips and slides are required but not supplied. They can be ordered from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Staining of fixed cells or tissue sectionsa. Wash cells or tissue sections twice with PBS.b. Fix cells or tissue sections at room temperature for 10-20 minutes with 's Immunol Staining Fix Solution or PBS containing 3.7% formaldehyde.c. Wash cells or tissue sections 2-3 times for 5 minutes each with 's Immunol Staining Wash Buffer or PBS containing 0.1% Triton X-100.d. Dilute Tubulin-Tracker Green in a 1:50-100 ratio with 's Immunol Fluorescence Staining Secondary Antibody Dilution Buffer or PBS containing 1-5% BSA and 0.1% Triton X-100. For example, dilute 4µl of Tubulin-Tracker Green in 0.2ml or 0.4ml of dilution buffer to obtain the Tubulin-Tracker Green working solution. The dilution ratio can be adjusted according to the staining result.e. Add 100µl of Tubulin-Tracker Green working solution onto each slide and incubate for 30-60 minutes at room temperature in the dark. To minimize evaporation, it is better to place the slide in a Staining Box for Microscope Slides (, FSR958).f. Wash the slides 2-4 times for 5 min each with 's Immunol Staining Wash Buffer or PBS containing 0.1% Triton X-100.g. The stained slide can be directly observed with a fluorescence microscope. The slides can also be observed or stored after mounting with anti-fade mounting medium.2. Staining of cells for flow cytometric analysis a. Collect approximately 0.2-0.5×106 cells per sample.b. Wash once with PBS.c. Fix the cells at room temperature for 10-20 minutes with 's Immunol Staining Fix Solution or PBS containing 3.7% formaldehyde.d. Wash the cells 2-3 times for 5 minutes each time with 's Immunol Staining Wash Buffer or PBS containing 0.1% Triton X-100.e. Resuspend cells with 's Immunol Fluorescence Staining Secondary Antibody Dilution Buffer or PBS containing 1-5% BSA and 0.1% Triton X-100, then apply Tubulin-Tracker Green at a ratio of 1:50-100. For example, add 1µl of Tubulin-Tracker Green to 50-100µl of cell resuspension. The dilution ratio of Tubulin-Tracker Green can be adjusted according to the staining results.f. Incubate for 1 hour at room temperature in the dark.g. Wash 2-3 times for 5 minutes each with 's Immunol Staining Wash Buffer or PBS containing 0.1% Triton X-100. h. Examine cells by Flow cytometry.

Specifications

Stability And Storage

Store at -20℃ in the dark for up to 1 year.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.