UltraBio™ DNA Size Selection Magnetic Beads

Cat. No.: D751553

Synonyms

DNA Fragselect XP Magnetic Beads

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Application

Nucleic Acid Extraction and Purification

Overview

UltraBio™ DNA Size Selection Magnetic Beads utilize Solid Phase Reverse Immobilization (SPRI) technology. These beads are coated with a novel nucleic acid purification medium and combined with an optimized buffer system. At specific bead-to-sample ratios, they enable the isolation and purification of nucleic acid fragments within defined size ranges. This product is designed for DNA fragment size selection and purification during next-generation sequencing (NGS) library preparation, allowing users to obtain DNA libraries of varying sizes by adjusting the sample loading conditions.

Instructions for Use:

I. User-Supplied Reagents and Equipment

1.80% Ethanol

2.Elution Buffer: 10 mM Tris-HCl (pH 8.0) or Nuclease-Free Water

3.Equipment: Pipettes, Vortex mixer, Magnetic rack, Microcentrifuge tubes (1.5 mL)

II. DNA Fragment Size Selection Procedure

1.First Binding:

Transfer the sample to a 1.5 mL microcentrifuge tube.

Add the specified volume of Bead Suspension according to Table 1 (Bead Suspension Volume = Sample Volume × First Binding Ratio).

Vortex-mix thoroughly. Incubate at room temperature for 10 minutes.

Place the tube on the magnetic rack until the solution clears completely.

Transfer the supernatant to a new 1.5 mL microcentrifuge tube. Discard the beads.

2.Second Binding:

Add the specified volume of Bead Suspension to the supernatant from Step 1 according to Table 1 (Bead Suspension Volume = Original Sample Volume × Second Binding Ratio).

Vortex-mix thoroughly. Incubate at room temperature for 10 minutes.

Place the tube on the magnetic rack until the solution clears completely. Discard the supernatant.

3.Wash 1:

With the tube on the magnetic rack, add 200 μL of 80% Ethanol. Do NOT resuspend the beads.

Incubate for 1 minute.

Carefully aspirate and discard the supernatant while keeping the tube on the magnetic rack.

4.Wash 2:

Repeat Step 3 once.

5.Ethanol Removal:

Keep the tube on the magnetic rack. Air-dry the beads at room temperature for 2-5 minutes.

Note: Avoid over-drying the beads, as this reduces purification efficiency.

6.Elution:

Remove the tube from the magnetic rack.

Add 30 μL Elution Buffer. Vortex-mix thoroughly to resuspend the beads.

Incubate at room temperature for 10 minutes.

7.Nucleic Acid Recovery:

Place the tube back on the magnetic rack for 2-5 minutes until the solution clears completely.

Carefully transfer the supernatant (containing the purified DNA) to a new tube.

Table 1: DNA Size Selection Ranges and Bead Suspension Addition Ratios

Size Selection Range	200-300bp	300-400bp	400-500bp	500-600bp	600-800bp
First Binding Ratio	0.80×	0.70×	0.60×	0.55×	0.50×
Second Binding Ratio	0.20×	0.20×	0.20×	0.15×	0.15×

III. PCR Product Purification Procedure

1.Binding:

Add an equal volume of Bead Suspension to the PCR product sample.

Vortex-mix thoroughly. Incubate at room temperature for 5 minutes.

Place the tube on the magnetic rack until the solution clears completely. Discard the supernatant.

2.Wash 1:

With the tube on the magnetic rack, add 200 μL of 80% Ethanol. Do NOT resuspend the beads.

Incubate for 1 minute.

Carefully aspirate and discard the supernatant while keeping the tube on the magnetic rack.

3. Wash 2:

Repeat Step 2 once.

Keep the tube on the magnetic rack. Air-dry the beads at room temperature for 2-5 minutes.

Note: Avoid over-drying the beads, as this reduces purification efficiency.

4.Ethanol Removal:

Keep the tube on the magnetic rack. Air-dry the beads at room temperature for 2-5 minutes.

Note: Avoid over-drying the beads, as this reduces purification efficiency.

5.Elution:

Remove the tube from the magnetic rack.

Add 20-100 μL Elution Buffer (user-defined volume). Vortex-mix thoroughly to resuspend the beads.

Incubate at room temperature for 5 minutes.

6.Nucleic Acid Recovery:

Place the tube back on the magnetic rack for 2-5 minutes until the solution clears completely.

Carefully transfer the supernatant (containing the purified PCR product) to a new tube.

Precautions:

1.Storage & Handling: This reagent is supplied as a Bead Suspension. DO NOT freeze or centrifuge the suspension. Vortex-mix thoroughly before initial use.

2.Sample Volume: Minimum recommended sample volume: ≥100 μL (to minimize pipetting error). For samples <100 μL, adjust to 100 μL using Nuclease-Free Water.

3.Pre-Use Equilibration: Equilibrate the Bead Suspension to room temperature (RT) for ≥30 minutes before use.

4.Bead Drying: Avoid over-drying the beads during the drying step, as this will significantly reduce elution efficiency.

Specifications

Synonyms

DNA Fragselect XP Magnetic Beads

Stability And Storage

Store at 2-8℃ long term (12 months). Do not freeze.

Storage

Store at 2-8°C, Do not freeze

Shipped In

Wet ice, Do not freeze

This product requires cold chain shipping. Ground and other economy services are not available.