UltraBio™ Plus Native-PAGE Hepes Electrophoresis Buffer

Cat. No.: P751567

GRADE & PURITY 20X

Overview

Precautions：

This product is suitable for Hepes buffered polyacrylamide gels. Do not use it for polyacrylamide gels of other buffer systems.The size of prestained protein ladders in Hepes electrophoresis system is slightly different from that in other electrophoresis systems such as Tris-Glycine electrophoresis system.This product does not contain SDS and is suitable for electrophoresis of native proteins. For electrophoresis of denatured proteins, we recommend using Plus SDS-PAGE Hepes Electrophoresis Buffer (20X) .The diluted electrophoresis buffer can usually be stored at room temperature for 2-3 days or at 4ºC for 1-2 weeks.The recovered electrophoresis buffer cannot be used in the inner chamber, but can be reused 1-2 times in the outer chamber. The use of electrophoresis buffer that has been reused for too many times or has been kept for too long may affect the electrophoresis results.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Prepare one liter of 1X Electrophoresis Buffer by diluting 50ml of Native PAGE Hepes Electrophoresis Buffer (20X) with 950ml of distilled or deionized water. It can be stored at room temperature for 2-3 days or at 4ºC for 1-2 weeks.2. For detailed Western operations, please refer to http://www.aladdin-e.com/support/western.htm. FAQ: 1. There are crystal precipitates in this product. This product is a 20X concentrated solution with high concentrations of salt. Precipitation may occur in this product at low temperatures. The precipitates should be dissolved completely in a 37ºC water bath prior to use.2. When electrophoresis at a constant 150V voltage, the current of each gel is between 110-130mA, which will gradually decrease over time. If the current is not within this range, check the quality of the electrophoresis buffer and whether there is any leakage of the electrophoresis buffer in the inner chamber.

Specifications

Product Name

UltraBio™ Plus Native-PAGE Hepes Electrophoresis Buffer

Specifications & Purity

20X

Molecule Type

Bioreagents/Buffer

Storage and Shipping

Concentration

20X

Storage

Room temperature

Shipped In

Normal

Stability And Storage

Store at room temperature for up to 1 year.