UltraBio™ Primary Antibody Dilution Buffer for Immunostaining

Cat. No.: P752165

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

To ensure the best results, we recommend using this product in conjunction with QuickBlock™ Blocking Buffer for Immunol Staining , and Immunol Staining Wash Buffer .A small amount of white precipitates may occur in this product after stored at low temperatures or frozen at -20°C. Before use, the precipitates should be completely dissolved by incubating the bottle in a 30-50°C water bath for an appropriate period of time.Primary antibodies diluted and prepared with this product can be stored at 4℃for multiple uses in immunostaining, but should be discarded when there is turbidity, precipitation, or a decrease of assay efficacy.Diluted antibodies should be stored at 4℃immediately after use to enable multiple uses.This product is recommended for primary antibody dilution, but not for sample blocking.For primary antibody dilution, there is no need to add other blocking reagents such as BSA, skim milk powder, and casein, as this product has been optimized to have good blocking effect. The addition of other blocking reagents may have some negative effects.The effect of this product varies for different target proteins and antibodies.If the signal of the target protein is very strong, the signal of non-specific bindings will be enhanced accordingly. If the non-specific signal intensity is very close to that of target protein, attempt to decrease the concentration of antibody or shorten the exposure time appropriately.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Dilute the primary antibody with this product at an appropriate ratio according to manufacture's instructions of the antibody used and the content of target protein in samples. The diluted primary antibody can be recovered and stored at 4℃for later use in immunostaining. 2. For detailed procedures of immunostaining, please refer to http://www.aladdin-e.com

Specifications

Stability And Storage

Store at 4℃ for up to 1 year, or -20℃ for long-term storage.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.