Urease (URH), CAS No.9002-13-5

CAS: 9002-13-5 Cat. No.: U754676 EC Number: 232-656-0

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Jack bean; ≥100 U/mg enzyme powder; ≥150 U/mg protein

Accession #

P07374

Bioactivity

≥100 U/mg enzyme powder; ≥150 U/mg protein

Overview

  Urease is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.Subunit molecular weight: ~90,770

  Composed of six subunits with total molecular weight: ~544,620

Application

  Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis.

Biochem/physiol Actions

  Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline.

Specifications

Product Name

Urease (URH), CAS No.9002-13-5

Synonyms

Urea amidohydrolase | acid urease | Urea amidohydrolas

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, from Jack bean; ≥100 U/mg enzyme powder; ≥150 U/mg protein

Biochemical and Physiological Mechanisms

Urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism. Is known to be highly toxic and lethal when given by intravenous route, producing convulsions and other signs of central nervous system intoxication associated wit

Bioactivity

≥100 U/mg enzyme powder; ≥150 U/mg protein

Accession #

P07374

CAS

9002-13-5

Enzyme Commission Number

EC 3.5.1.5

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Jack bean; ≥100 U/mg enzyme powder; ≥150 U/mg protein

Reconstitution

Reconstitute in water to a concentration of 10 mg/ml.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ short term (12 months). Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

1 U Corresponds to the Amount of Enzyme which Hydrolyzes 1 µmol Urea per Minute at ph 8.0 and 25 deg C

References

1. Xinyu Shao, Lulu Cao, Lixia Lu.  (2023)  Ultrasensitive detection of glucose oxidase and alkaline phosphatase in milk based on valence regulated upconversion nanoprobes.  FOOD CHEMISTRY,      [PMID:37634343] [10.1016/j.foodchem.2023.137212]

2. Qin Yang, Zhenkun Zhang, Lin Wang, Xiwen Xing, Jiahai Zhou, Long Li.  (2023)  Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening.  Nanomaterials,  13  (14): (2064).  [PMID:37513075] [10.3390/nano13142064]

3. Zhao Chang, Xiao Yang, Chu Jian, Hu Ran, Liu Hanlong, He Xiang, Liu Yi, Jiang Xiang.  (2023)  Microfluidic experiments of biological CaCO3 precipitation in transverse mixing reactive environments.  Acta Geotechnica,  18  (10): (5299-5318).  [PMID:] [10.1007/s11440-023-01938-w]

4. Pengyu Xi, Xuecheng He, Chuan Fan, Qinglin Zhu, Zehua Li, Yuemeng Yang, Xin Du, Tailin Xu.  (2023)  Smart Janus fabrics for one-way sweat sampling and skin-friendly colorimetric detection.  TALANTA,      [PMID:37058940] [10.1016/j.talanta.2023.124507]

5. Liangliang Cheng, Wanlu Zheng, Ya-Nan Zhang, Xuegang Li, Yong Zhao.  (2023)  Highly Sensitive Fiber-Optic SPR Urea Sensor Based on ZIF-8/Urease.  IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT,      [PMID:] [10.1109/TIM.2023.3259040]

6. Yuyang Bei, Kamile Arkin, Yuxin Zheng, Xuesong Ma, Jie Zhao, Huimin Jin, Qingkun Shang.  (2022)  Construction of a ratiometric fluorescent probe for visual detection of urea in human urine based on carbon dots prepared from Toona sinensis leaves and 5-carboxyfluorescein.  ANALYTICA CHIMICA ACTA,      [PMID:36641152] [10.1016/j.aca.2022.340733]

7. Wanying Cui, Chunmiao Xia, Sheng Xu, Xinke Ye, Yihao Wu, Shukai Cheng, Rongli Zhang, Cuige Zhang, Zongcheng Miao.  (2022)  Water-in-water emulsions stabilized by self-assembled chitosan colloidal particles.  CARBOHYDRATE POLYMERS,      [PMID:36657862] [10.1016/j.carbpol.2022.120466]

8. Xuecui Mei, Jiao Yang, Jiang Liu, Yingchun Li.  (2022)  Wearable, nanofiber-based microfluidic systems with integrated electrochemical and colorimetric sensing arrays for multiplex sweat analysis.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2022.140248]

9. Ming Zhao, Zhenhai Chen, Li Hao, Huayao Chen, Xinhua Zhou, Hongjun Zhou.  (2022)  CMC based microcapsules for smart delivery of pesticides with reduced risks to the environment.  CARBOHYDRATE POLYMERS,      [PMID:36372488] [10.1016/j.carbpol.2022.120260]

10. Lingxia Qin, Xinxin Ren, Kaiyue Hu, Di Wu, Zhiyong Guo, Sui Wang, Linwen Jiang, Yufang Hu.  (2022)  Supramolecular Host-Guest Interaction-Driven Electrochemical Recognition for Pyrophosphate and Alkaline Phosphatase Analysis.  CHEMBIOCHEM,  23  (20): (e202200413).  [PMID:35997506] [10.1002/cbic.202200413]

11. Deng Hao-Hua, Yang Hui-Jing, Huang Kai-Yuan, Zheng Yi-Jing, Xu Ying-Ying, Peng Hua-Ping, Liu Yin-Huan, Chen Wei, Hong Guo-Lin.  (2022)  Antenna effect of pyridoxal phosphate on the fluorescence of mitoxantrone-silicon nanoparticles and its application in alkaline phosphatase assay.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  414  (17): (4877-4884).  [PMID:35576012] [10.1007/s00216-022-04110-7]

12. Yuan Yuan, Yuhan He, Dongni Pei, Liujuan Tong, Shengqiang Hu, Lin Liu, Xinyao Yi, Jianxiu Wang.  (2022)  Urease-Functionalized Near-Infrared Light-Responsive Gold Nanoflowers for Rapid Detection of Urea by a Portable Pressure Meter.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2022.107450]

13. Fei Qu, Zhuo Wang, Cong Li, Dafeng Jiang, Xian-en Zhao.  (2022)  Peptide cleavage-mediated aggregation-enhanced emission from metal nanoclusters for detecting trypsin and screen its inhibitors from foods.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2022.131610]

14. Huan Wang, Hui Zhang, Dagan Zhang, Jie Wang, Hui Tan, Tiantian Kong.  (2021)  Enzyme-functionalized structural color hydrogel particles for urea detection and elimination.  Journal of Cleaner Production,      [PMID:] [10.1016/j.jclepro.2021.128149]

15. Qiaozhen Bao, Dan Lin, Yaoran Gao, Lina Wu, Jinhua Fu, Khuslen Galaa, Xinhua Lin, Liqing Lin.  (2021)  Ultrasensitive off-on-off fluorescent nanosensor for protamine and trypsin detection based on inner-filter effect between N,S-CDs and gold nanoparticles.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2021.106409]

16. Zhanlin Zhang, Dandan Zhang, Bo Qiu, Wenxiong Cao, Yuan Liu, Qingjie Liu, Xiaohong Li.  (2021)  Icebreaker-inspired Janus nanomotors to combat barriers in the delivery of chemotherapeutic agents.  Nanoscale,  13  (13): (6545-6557).  [PMID:33885534] [10.1039/D0NR08853F]

17. Zhanlin Zhang, Hui Yan, Shang Li, Yuan Liu, Pan Ran, Weijia Chen, Xiaohong Li.  (2020)  Janus rod-like micromotors to promote the tumor accumulation and cell internalization of therapeutic agents.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2020.127073]

18. Jue Zhang, Bingqing Yan, Chao He, Yuanyuan Hao, Shudong Sun, Weifeng Zhao, Changsheng Zhao.  (2020)  Urease-Immobilized Magnetic Graphene Oxide as a Safe and Effective Urea Removal Recyclable Nanocatalyst for Blood Purification.  INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,      [PMID:] [10.1021/acs.iecr.0c00302]

19. Jue Zhang, Zhenqiang Shi, Chao He, Xin Song, Ye Yang, Shudong Sun, Weifeng Zhao, Changsheng Zhao.  (2019)  Urease immobilized GO core@shell heparin-mimicking polymer beads with safe and effective urea removal for blood purification.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:31783078] [10.1016/j.ijbiomac.2019.11.197]

20. Zhoujiang Chen, Tian Xia, Zhanlin Zhang, Songzhi Xie, Tao Wang, Xiaohong Li.  (2019)  Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2019.122109]

21. Yang Hu, Yan Sun.  (2019)  Autonomous motion of immobilized enzyme on Janus particles significantly facilitates enzymatic reactions.  BIOCHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.bej.2019.107242]

22. Qu Fei, Ding Yanru, Lv Xiaoxia, Xia Lian, You Jinmao, Han Wenli.  (2019)  Emissions of terbium metal–organic frameworks modulated by dispersive/agglomerated gold nanoparticles for the construction of prostate-specific antigen biosensor.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,  411  (17): (3979-3988).  [PMID:31089787] [10.1007/s00216-019-01883-2]

23. Muhammad Bilal, Hafiz M.N. Iqbal.  (2019)  Naturally-derived biopolymers: Potential platforms for enzyme immobilization.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:30825566] [10.1016/j.ijbiomac.2019.02.152]

24. Jue Zhang, Zhoujun Wang, Chao He, Xiaoling Liu, Weifeng Zhao, Shudong Sun, Changsheng Zhao.  (2019)  Safe and Effective Removal of Urea by Urease-Immobilized, Carboxyl-Functionalized PES Beads with Good Reusability and Storage Stability.  ACS Omega,      [PMID:] [10.1021/acsomega.8b03287]

25. Hao-Hua Deng, Hua-Ping Peng, Kai-Yuan Huang, Shao-Bin He, Qiao-Feng Yuan, Zhen Lin, Rui-Ting Chen, Xing-Hua Xia, Wei Chen.  (2019)  Self-Referenced Ratiometric Detection of Sulfatase Activity with Dual-Emissive Urease-Encapsulated Gold Nanoclusters.  ACS Sensors,      [PMID:30652857] [10.1021/acssensors.8b01130]

26. Li Fu, Yong Huang, Wen Cai, Huaiwei Zhang, Jiehui Yang, Dihua Wu, Weitao Su.  (2018)  Improving the hydrophilicity and chronocoulometric performance of TiO2 nanotubular arrays by Sr@Si doping.  CERAMICS INTERNATIONAL,      [PMID:] [10.1016/j.ceramint.2018.07.256]

27. Jing Ouyang, Dawei Mu, Yi Zhang, Huaming Yang, Steven L. Suib.  (2018)  Selective Fabrication of Barium Carbonate Nanoparticles in the Lumen of Halloysite Nanotubes.  Minerals,  8  (7): (296).  [PMID:] [10.3390/min8070296]

28. Wenyue Xie, Lingli Lei, Meiling Tian, Zeying Zhang, Yingshuai Liu.  (2018)  A high-resolution colorimetric immunoassay platform realized by coupling enzymatic multicolor generation with smartphone readout.  ANALYST,  143  (12): (2901-2907).  [PMID:29808208] [10.1039/C8AN00382C]

29. Lv Miaomiao, Ma Xiaofei, Anderson Debbie P., Chang Peter R..  (2017)  Immobilization of urease onto cellulose spheres for the selective removal of urea.  CELLULOSE,  25  (1): (233-243).  [PMID:] [10.1007/s10570-017-1592-3]

30. Mengke Wang, Lei Wang, Qing Liu, Xingguang Su.  (2017)  A fluorescence sensor for protein kinase activity detection based on gold nanoparticles/copper nanoclusters system.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2017.09.213]

31. Dong-Sheng Zhao, Li-Long Jiang, Ya-Xi Fan, Ling-Li Wang, Zhuo-Qing Li, Wei Shi, Ping Li, Hui-Jun Li.  (2017)  Investigation of Dioscorea bulbifera Rhizome-Induced Hepatotoxicity in Rats by a Multisample Integrated Metabolomics Approach.  CHEMICAL RESEARCH IN TOXICOLOGY,      [PMID:28899093] [10.1021/acs.chemrestox.7b00176]

32. Kai Huang, Tao Zhang, Bo Jiang, Wanmeng Mu, Ming Miao.  (2017)  A coupled system involving arginase and urease for l-ornithine production.  JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,      [PMID:] [10.1016/j.molcatb.2017.01.018]

33. Maohua Yang, Liangli Li, Chunni Zhao, Mei Zhang, Mingyan Jia.  (2024)  A colorimetric sensor array for the rapid identification of benzylisoquinoline alkaloids and Coptidis Rhizoma based on urease inhibition.  FOOD CHEMISTRY,      [PMID:39755044] [10.1016/j.foodchem.2024.142610]

34. Hui Liu, Yi Lu, Bitao Peng, Jingxian Wei, Hongli Dai, Lei Wang, Hongxiang Zhu, Hui He.  (2025)  Cellulose self-erasing material with dual functions in information encryption.  CARBOHYDRATE POLYMERS,      [PMID:40158981] [10.1016/j.carbpol.2025.123444]

35. Long Chen, Liang Li, Hongyan Chen, Bo Cui, Fei Gao, Jinming Gao, Xiang Zhao, Yan Wang, Haixin Cui, Zhanghua Zeng.  (2024)  Chitin nanocrystal Pickering emulsions for sustainable release pesticide microencapsulation with superior leaf adhesion and enhanced safety.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2024.157452]

36. Lei Zhou, Dian Wang, Jiayu Yu, Hongyi Zhang, Hang Dong.  (2025)  CO2-driven ion exchange for ammonium recovery from source-separated urine.  WATER RESEARCH,      [PMID:39817966] [10.1016/j.watres.2025.123132]

37. Yihong Chen, Lili Yin, Hao Dong, Yang Hu, Xiangzhao Mao.  (2024)  Enhanced enzymatic degradation of viscous marine polysaccharide by the self-propulsion of urease-driven micromotor.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2024.158988]

38. Peng Han, Yuan Li, Bin Zhao, Huiliang Li, Zheng Wang, Xue Liu, Weihua Meng.  (2025)  Visual and label-free detection of urea based on amorphous photonic films with non-iridescent structural colors.  ANALYTICA CHIMICA ACTA,      [PMID:40015773] [10.1016/j.aca.2025.343731]

39. Chenwei Sun, Guohua Wu, Di Wu, Lin Wu, Qingrui Lu, Wenqi Hu, Qijun Du, Jiashu Wang, Ao Xie, Mengjiao Xia, Haijie Hu, Bangchuan Hu, Jian Huang, Shuqi Wang.  (2025)  Determining the Optimal Transplantation Window in Hepatic Organoids via Real-Time Biosensing of Vascularization and Metabolic Maturation Utilizing the Integrated Organoid-on-a-Chip Platform.  BIOSENSORS & BIOELECTRONICS,      [PMID:41046609] [10.1016/j.bios.2025.118057]

40. Jian-Qing Liu, Ye-Bin Feng, Yin-Feng Xiao, Lu-Fang Chen, You-Xia Huang, Shui-Sen Lin, Yin Zhang, Qiu-Xia Xu, Shao-Bin He, Wei Chen.  (2025)  High-performance glucose isomerase detection enabled by self-cascade copper oxide nanozyme system.  TALANTA,      [PMID:40819506] [10.1016/j.talanta.2025.128705]

41. Yahui Guo, Xin Yang, Cui Liao.  (2025)  Design of Boron Nitride Nanosheet-Based Electrochemical Biosensors for Urea Detection in Athlete Biological Fluids.  ELECTROCHIMICA ACTA,      [PMID:] [10.1016/j.electacta.2025.147536]

42. Li Ming-Yan, Yu Yaqi, Zhang Rui-Qiao, Qiu Zhiwei, Li Xiang, Wang Ying-Hui, Qi JiaYi, Zeng Jingbin, Wen Cong-Ying.  (2025)  Au@Ag@AgCl core-shell nanostructure-based label-free biosensor for sensitive colorimetric detection of urease.  MICROCHIMICA ACTA,  192  (11): (1-10).  [PMID:41175148] [10.1007/s00604-025-07649-1]

43. Qizhu Qian, Qilong Liu, Mengxiang Wang, Jingjing Yang, Huiyi Li, Wei Bai, Wentao Wang, Changzheng Wu, Chong Xiao, Yi Xie.  (2025)  Dual-site cooperation for synergistic optimization of the band structure and spin state to facilitate C–N coupling reaction.  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,  122  (43): (e2508077122).  [PMID:41118215] [10.1073/pnas.2508077122]

44. Gao Haonan, Wen Mingzhen, Li Jiali, Hou Shili, Li Lifen, Liu Guangyan.  (2026)  Electrodeposition preparation of Ni/Co-MOF/MXene heterojunction to fabricate enzymatic electrochemical biosensor for detecting trace urea.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,      [PMID:41723337] [10.1007/s00216-026-06407-3]

45. Kermue Vasco Jarnda, Heng Dai, Huiting Hu, Yuan Tian, Richmond Anaman, Pian Wu, Ping Ding.  (2026)  Construction of a Portable Colorimetric Biosensing Platform Based on the Co-TCPP (Fe)-MOF Nanosheet and Its Application in Glucose Detection.  ACS Omega,      [PMID:41835601] [10.1021/acsomega.5c10036]