BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Protected from light,Room temperature Ships Normal Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
When proteins are far from their isoelectric point, they tend to form insoluble precipitates. Sulfosalicylic acid is a biological alkaloid reagent. Under acidic conditions, its anions can combine with positively charged proteins to form insoluble protein salts, resulting in precipitation. This product is a qualitative detection kit with a sensitivity of 0.05-0.1 g/L, primarily used for detecting proteins in human or animal urine. This kit is intended for research use only and is not suitable for clinical diagnosis or other purposes
| U1506746 | Component | 100T | Storage |
| U1506746A | Sulfosalicylic Acid Solution | 10 mL | RT. Store in the dark. |
| U1506746B | Acidification Solution | 10 mL | RT. |
Experimental Procedure
Transfer 3-5 ml of fresh urine into a small test tube.
Add 1-2 drops of Sulfosalicylic Acid Solution, allowing it to form a layer/interface.
Observe immediately. If turbidity appears, it indicates the presence of protein in the urine. The depth of turbidity corresponds to the approximate concentration.
Result Interpretation
| Result Interpretation | Phenomenon Description | Estimated Protein Concentration |
| Negative (-) | No turbidity, clear and transparent | < 0.05 g/L |
| Suspicious (±) | Slight turbidity, faintly visible | 0.05 - 0.2 g/L |
| (+) | Distinct white turbidity, no granules | ~ 0.3 g/L |
| (2+) | Thin, milky turbidity with appearing granules | ~ 1 g/L |
| (3+) | Milky and opaque, with flocculent precipitates | ~ 3 g/L |
| (4+) | Flocculent precipitates, coagulated into clumps | > 5 g/L |
Precautions
If the urine sample is turbid, it should be centrifuged or filtered first.
Highly alkaline urine may cause false-positive results.
The sensitivity of this method is 0.05-0.1 g/L.
Highly alkaline urine may also lead to false-negative results. Add a few drops of the Acidification Solution and retest.
False-positive results can be caused by organic contrast agents, large doses of penicillin, or high concentrations of uric acid/urates in the urine.
High concentrations of urea or urates in the urine may cause false-positive results.
Use reagents promptly after opening to prevent potential effects on subsequent experimental results.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View BioReagent grade guide →
