Urobilinogen Qualitative Detection Kit (Modified Ehrlich's Method)

Cat. No.: U1509467

GRADE & PURITY BioReagent

Overview

Urobilinogen, also known as urochrome, is formed when aged red blood cells are broken down in the liver or spleen. Hemoglobin is converted into indirect bilirubin, which is then metabolized by intestinal bacteria into urobilinogen.

Detection Principle

Under acidic conditions, Ehrlich's reagent reacts with urobilinogen to produce a red-colored compound. This kit is highly sensitive and is primarily used for the qualitative detection of urobilinogen in human or animal urine. This kit is for research use only and is not suitable for clinical diagnosis or other purposes.

Reagents, consumables and Equipments not provided

• Centrifuge
• Test tubes or centrifuge tubes
• Chloroform
  

Operating Steps (For Reference Only)

1. (Optional) If the urine sample contains bilirubin, it should be removed. Take 4 mL of fresh urine into a test tube, add approximately 1 mL of Barium Chloride Solution, mix well, centrifuge at 3000 g for 5 minutes, discard the precipitate, and keep the supernatant for use.

2. Take 2 mL of fresh, bilirubin‑free urine, add 0.2 mL of Ehrlich's Reagent, let stand for 10 minutes, and observe the result.

Result Interpretation

Observation method: Observe from the top of the tube against a white background, looking directly to the bottom.

Result	Observation
Negative	No red color appears.
Positive (+)	Appears faint red.
Positive (2+)	Appears cherry red.
Positive (3+)	Immediately appears deep red.

Note: Normal urine should give a positive (+) result.

Precautions

1. Urine must be fresh. Urobilinogen oxidizes to urobilin upon prolonged standing, leading to false‑negative results.

2. If the urine contains interfering substances such as porphobilinogen, add 2 mL of chloroform after adding Ehrlich's Reagent. Shake well and let stand. Urobilinogen will transfer to the chloroform layer, confirming a positive result.

3. If antibiotics have suppressed the intestinal flora, urobilinogen may be reduced or absent.

4. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.

5. For your safety and health, please wear a lab coat and disposable gloves during operation.

Storage and Shipping

Storage

Protected from light,Room temperature

Shipped In

Normal

Stability And Storage

Store at room temperature long term (12 months). Store in the dark.

Contents & Storage

U1509467	Component	100T	Storage
U1509467A	Barium Chloride Solution	50 mL	RT.
U1509467B	Ehrlich's Reagent	20 mL	RT. Store in the dark.