Wheat Germ Agglutinin (WGA), TRITC conjugate, CAS No.96508-08-6

CAS: 96508-08-6 Cat. No.: np231289

GRADE & PURITY BioReagent, Suitable for Immunofluorescence(IF), 1.0 mg/mL

Overview

  Triticum vulgaris lectin or Wheat germ agglutinin (WGA) is isolated from Triticum vulgaris (Wheat germ) and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 36 kDa. WGA selectively binds to N-Acetyl glucosamine (GlcNAc) and to N-acetylneuraminic acid (sialic acid) residues of glycoproteins and glycolipids, which are often found on cell membranes.

  WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections, or for western blotting.  WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae.

TRITC labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 557 nm and the emission maximum is at 576nm. Fluorescent WGA conjugates provide selective labeling of the plasma membrane with minimal background in many cell types that is retained after formaldehyde fixation and permeabilization with Triton X-100.

Specifications

Product Name

Wheat Germ Agglutinin (WGA), TRITC conjugate, CAS No.96508-08-6

Synonyms

WGA, TRITC conjugate | Lectin from Triticum vulgaris (wheat), TRITC conjugate | Rhodamine WGA | Rhodamine Wheat Germ Agglutinin | Wheat Germ Agglutinin (WGA), TRITC | TRITC, WGA | TRITC-Wheat germ agglutinin | WGA-TRITC

Grade

BioReagent, Suitable for Immunofluorescence(IF)

Specifications & Purity

BioReagent, Suitable for Immunofluorescence(IF), 1.0 mg/mL

Excitation (nm)

Green Laser(532nm)

Emission(nm)

576nm

Ex/Em(nm)

CAS

96508-08-6

Molecule Type

Protein

Storage and Shipping

Concentration

1.0 mg/mL

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Images

IF/ICC: Wheat Germ Agglutinin (WGA), TRITC conjugate (np231289)

Wheat Germ Agglutinin (WGA), TRITC conjugate (np231289) - IF/ICC 

HeLa cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Wheat Germ Agglutinin (WGA), TRITC conjugate (np231289) (red) at 1/200 dilution in blocking buffer overnight at 4°C in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

References

1. Qian Wang, Zichao Peng, Bin Gui, Yongming Li, Lanjie Liao, Zuoyan Zhu, Yaping Wang, Libo He.  (2025)  Sialic acid serves as a functional receptor for grass carp reovirus.  PLoS Pathogens,  21  (9): (e1013481).  [PMID:40911632] [10.1371/journal.ppat.1013481]

2. Donghua Su, Xiaolong Zhang, Tianwu An, Xi Wang, Yaqiu Lin, Haibo Wang, Peiqiang Yu, Haitao Shi.  (2025)  Astral-DIA proteomics and 4D label-free N-glycoproteomics of milk fat globule membrane proteins reveal distinct profiles between yak colostrum and mature milk.  Food Chemistry-X,      [PMID:41378274] [10.1016/j.fochx.2025.103302]