Wright stain is a compound dye composed of the acidic dye Eosin and the basic dye Methylene Blue, which delivers excellent differential staining of protoplasm. Giemsa stain is a mixture of Azure II and Eosin. Its staining principle and resultant coloration are basically consistent with Wright staining. Giemsa stain exhibits strong affinity for cytoplasm and clearly reveals the basophilic degree of cytoplasm. In particular, it distinctly stains azurophilic, eosinophilic and basophilic granules in blood and bone marrow cells. However, it overstains cell nuclei and fails to display fine nuclear structures clearly. For this reason, Giemsa stain is frequently used in combination with Wright stain.
Wright-Giemsa Staining Kit is formulated with high-quality Wright and Giemsa dyes as the primary raw materials, yielding sharp and clear cellular staining results. It is widely applied for staining blood smears, cell smears, bone marrow smears and bacteria. Cytoplasm stains red; cell nuclei and bacteria appear blue; eosinophilic granules turn orange-red. Neutral glycerol is added to the staining solution to inhibit methanol volatilization and oxidation, while also sharpening the staining morphology of blood cells. Features of this stain: The kit consists of Wright-Giemsa combined staining solution and phosphate buffer. The two components can be mixed in equal volumes for use, or applied to specimens separately.
M743374
| Component
| 2×100 mL
| 2×500 mL
| Storage
|
M743374A
| Wright-Giemsa Staining Solution
| 100 mL
| 500 mL
| RT. Store in the dark
|
M743374B
| Phosphate Buffer Solution
| 100 mL
| 500 mL
| RT. |
Instructions for Use:
1. Prepare blood smears, bone marrow smears or bacterial smears via standard protocols and allow the smears to air-dry naturally.
2. Place the blood or bone marrow smears on a staining rack.
3. Add an appropriate amount of Wright-Giemsa staining solution to fully cover the smear, and stain at room temperature for 1–2 minutes.
4. Add an equal volume of phosphate buffer solution to the smear. Gently rock the slide or mix by other means to fully blend the buffer with the Wright-Giemsa staining solution, then incubate at room temperature for 3–10 minutes.
5. Alternative procedure for Steps 3 and 4: Mix equal volumes of Wright-Giemsa staining solution and phosphate buffer to prepare Wright-Giemsa working solution. Apply the working solution to blood or bone marrow smears and incubate at room temperature for 3–10 minutes.
6. Gently rinse the slide from one end with tap water or distilled water. (Note: The slide can also be rinsed with phosphate buffer diluted at an equal ratio for approximately 30 seconds.)
7. Air-dry the slide. Microscopic examination: Observe the blood smear under low magnification first, then switch to oil immersion objective.
Precautions:
1. Blood and bone marrow smears must be uniformly thick to avoid uneven staining results.
2. Do not pour off the staining solution in advance or flush the smear with strong water flow during staining. Premature draining of the stain will cause dye precipitate to deposit on the smear.
3. The staining solution may be reused a limited number of times. Filter the solution to remove precipitates before reusing.
4. Over-stained slides can be appropriately decolorized with methanol or ethanol; re-staining is not recommended.
5. Adjust the staining duration or working solution concentration if staining appears too dark or too pale.
6. Wear a lab coat and disposable gloves during all operations for personal safety and health protection.
Staining Results:
Bacteria, Cell nuclei
| Blue
|
Cytoplasm of tissue cells, hemoglobin, eosinophilic granules
| Pink or orange-re |
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