GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of
Especies hospedadoras
Mouse
Especificidad
ACE2
Inmunógeno
Recombinant human ACE2 (AA 21-160).
Control positivo
WB: Calu-3 lysates. IHC: Human kidney tissue.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Mouse IgG1
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -80°C,Avoid repeated freezing and thawing
Enviado en
Dry ice packs + Cold packs
Estabilidad y almacenamiento
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
ACE2 Mouse mAb (Ab087119) - Western Blot 15 µg of Calu-3 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. ACE2 Mouse mAb (Ab087119) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. ACE2 band was visualized using ECL Western Blotting Substrate. Result: ACE2 Mouse mAb (Ab087119) can detect ACE2 by Western blotting.
ACE2 Mouse mAb (Ab087119) - IHC IHC analysis of kidney tissue by ACE2 Mouse mAb (Ab087119). IHC was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with ACE2 Mouse mAb (Ab087119) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Microvillus of cells in tubules are positively stained.
Aplicación
Aplicación
Dilution info
WB
1/1000
IHC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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