Annexin V/ANXA5 Mouse mAb - Primary antibody, specific to ANXA5, Mouse IgG1

Cat. No.: Ab088956

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, See COA

Application

Apoptosis, IF/ICC, IHC, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

C13

Conjugation

Unconjugated

Descripción general

Mouse anti Human Annexin V/ANXA5 Antibody, Monoclonal (C13), could be used for WB, IHC and so on. 

Specifications

Product Name

Annexin V/ANXA5 Mouse mAb - Primary antibody, specific to ANXA5, Mouse IgG1

Sinónimos

Anchorin CII | annexin A5 | Annexin V | annexin-5 | ANX5 | ANXA5 | ANXV | Calphobindin I | Endonexin II | ENX2CBP-I | Lipocortin V | PAP-I | Placental anticoagulant protein 4 | Placental anticoagulant protein I | PP4 | Thromboplastin inhibitor | VAC-alpha

Especificaciones y pureza

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Mecanismos bioquímicos y fisiológicos

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

Especies hospedadoras

Mouse

Especificidad

ANXA5

Inmunógeno

Recombinant ANXA5 expressed in E.coli (AA 1-320)

Control positivo

WB: HeLa, Hep G2, K562, HEK293, MDA-MB-231 and MCF7 cell lysates. IHC: Human small intestine, Human kidney, Human cerebrum, Human spleen, Human placenta, Human colon tissues. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A + Protein G purified

Concentración

See COA

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - Western Blot
All lanes: Annexin V/ANXA5 Mouse mAb (Ab088956) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 33 kDa
Exposure time: 1.5 s

IHC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Cerebrum Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 40 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Colon Tissue
Primary Ab: 20 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Cerebrum Tissue
Primary Ab: 20 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Small intestine Tissue
Primary Ab: 20 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 µg/mL Annexin V/ANXA5 Mouse mAb (Ab088956)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IF/ICC: Annexin V/ANXA5 Mouse mAb (Ab088956)

Annexin V/ANXA5 Mouse mAb (Ab088956) - IF/ICC
IF analysis of Annexin V/ANXA5 Mouse mAb (Ab088956) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Annexin V/ANXA5 Mouse mAb (Ab088956) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Aplicación

• Aplicación: Dilution info
• WB:

  0.01-4 µg/mL;

• IHC:

  2-40µg/mL ;

• IF/ICC:

  5 µg/mL;

Objetivos asociados (humanos)

ANXA5 Tbio Annexin A5 (0 Activities)

Discover Target: ANXA5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)