Carnoy Fixative Ⅱ, also known as Carnoy fixative, is mainly composed of ethanol, acetic acid and other components, and contains no chloroform. It is especially suitable for fixation of general tissues and cells with extremely rapid penetration capacity, and the fixation duration shall not exceed 24 h. It can quickly penetrate cells for fixation and maintain the integrity of chromosomal structure. It can also enhance the basophilia of chromosomes to achieve excellent staining results. If the fixed tissue is not for immediate use, it can be stored in 70% ethanol.
Storage: Store at 10–30 °C, protected from light; valid for 12 months.
Instructions for Use
1. Fixation of small tissue blocks: 30–40 min.
2. Larger tissue blocks: 2–4 h.
3. For bulky tissue samples, fixation time may be extended appropriately, but it should not exceed 24 h.
Precautions
1. Carnoy Fixative Ⅱ is potentially harmful to the human body. Handle with care. It is not suitable for fixation of tissues intended for lipid staining.
2. Fixation time varies with the thickness of harvested tissue.
3. Temperature has a marked effect on fixation. Elevated temperature accelerates fixation, but excessive temperature should be avoided.
4. Fresh tissue should be fixed immediately after sampling. If immediate fixation is unavailable, store the tissue in normal saline and submit for testing promptly.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
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