Cyclophilin B Mouse mAb (Biotin) - Primary antibody, specific to Cyclophilin B

Cat. No.: Ab219151

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

EF01/1F1

Conjugation

Biotin

Descripción general

Mouse anti Human Cyclophilin B Antibody, Monoclonal (EF01/1F1), could be used for WB and IF/ICC.

Specifications

Product Name

Cyclophilin B Mouse mAb (Biotin) - Primary antibody, specific to Cyclophilin B

Sinónimos

AA408962 antibody | AA553318 antibody | AI844835 antibody | Cphn 2 antibody | Cphn2 antibody | Cyclophilin B antibody | Cyclophilin like protein antibody | CyP 20b antibody | CYP S1 antibody | CYP-S1 antibody | CYPB antibody | EC 5.2.1.8 antibody | MGC141

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Especies hospedadoras

Mouse

Especificidad

Cyclophilin B

Inmunógeno

Recombinant protein corresponding to Human Cyclophilin B

Control positivo

WB: HeLa, K562 whole cell lysates IF/ICC: Hela cells

Conjugación

Biotin

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Estabilidad y almacenamiento

Store at -20°C. Protect from light. Avoid freeze/thaw cycles.

Imágenes

ICC/IF: Cyclophilin B Mouse mAb (Biotin) (Ab219151)

Cyclophilin B Mouse mAb (Biotin) (Ab219151) - ICC/IF
IF analysis of Cyclophilin B (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Cyclophilin B Mouse mAb (Biotin) (Ab219151) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (Oregon Green 488) (rp217313) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Western Blot: Cyclophilin B Mouse mAb (Biotin) (Ab219151)

Cyclophilin B Mouse mAb (Biotin) (Ab219151) - Western Blot
All lanes: Cyclophilin B Mouse mAb (Biotin) (Ab219151) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Streptavidin (HRP) (np156148) at 1/10000 dilution

Predicted band size: 18 kDa
Observed band size: 20 kDa
Exposure time: 100.0 s

Aplicación

• Aplicación: Dilution info
• WB:

  1/1000 - 1/5000 ;

• IF/ICC:

  1/100-1/1000 ;

Objetivos asociados (humanos)

PPIB Tchem Peptidyl-prolyl cis-trans isomerase B (0 Activities)

Discover Target: PPIB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)