GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Especies hospedadoras
Mouse
Especificidad
Human Cytokeratin 20
Inmunógeno
Recombinant protein corresponding to Human Cytokeratin 20
Control positivo
WB: HT-29 whole cell lysates IF/ICC: MCF-7 cells
Conjugación
Unconjugated
Grado
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Tipo de cadena ligera
kappa
SDS-PAGE
150.0 KDa
Método de purificación
Protein A purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Imágenes
Cytokeratin 20 Mouse mAb (Ab212580) - Western Blot All lanes: Cytokeratin 20 Mouse Antibody (Ab212580) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 44,47 kDa Exposure time: 10.0 s
Cytokeratin 20 Mouse mAb (Ab212580) - ICC/IF IF analysis of Cytokeratin 20 (Green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Cytokeratin 20 Mouse mAb (Ab212580) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Aplicación
Aplicación
Dilution info
IF/ICC
1/200
WB
1/1000 - 1/20000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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