GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, validado, 0,5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Conservar a -20°C,Evitar la congelación y descongelación repetidas Ships Hielera + almohadillas de hielo Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Anticuerpo monoclonal de ratón anti EIF2S1 humano (2E4F5E7), puede utilizarse para WB, IF/ICC, etc.
Specifications
Product Name
EIF2S1 ratón mAb - Primary antibody, Mouse IgG1
Sinónimos
Factor de iniciación de la traducción eucariótica 2 subunidad 1 | eIF-2-alfa | eIF-2A | EIF2A | CDA 02 | CDA02 | eIF 2 alfa | EIF 2 | EIF 2A | EIF-2alfa | EIF2 | EIF2alpha | Factor de iniciación de la traducción eucariótica 2 alfa | IF2A_HUMAN antibody |
Especificaciones y pureza
ExactAb™, validado, 0, 5 mg/mL
Mecanismos bioquímicos y fisiológicos
Funciona en los primeros pasos de la síntesis de proteínas formando un complejo ternario con GTP y ARNt iniciador. Este complejo se une a una subunidad ribosómica 40S, seguido de la unión del ARNm para formar un complejo de preiniciación 43S. Unión de la
Especies hospedadoras
Mouse
Inmunógeno
Purified recombinant human eIF2α protein fragments expressed in E.coli.
Control positivo
WB: K562, HeLa, MCF7, C2C12, Hep G2, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
Conjugación
Unconjugated
Grado
ExactAb™, Validated
Isotipo
Mouse IgG1
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Líquido
Condiciones de almacenamiento de almacenamiento
Conservar a -20°C,Evitar la congelación y descongelación repetidas
Enviado en
Hielera + almohadillas de hielo
Estabilidad y almacenamiento
Almacenar a 4℃ a corto plazo (1-2 semanas). Almacene en -20℃ a largo plazo (12 meses). Tras la recepción, se recomienda alícuota. Evite el ciclo de congelación/descongelación.
Imágenes
EIF2S1 Mouse mAb (Ab101289) - Western Blot All lanes: EIF2S1 Mouse mAb (Ab101289) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 10.0 s
EIF2S1 Mouse mAb (Ab101289) - IF/ICC Immunofluorescent analysis of EIF2S1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with EIF2S1 Mouse mAb (Ab101289) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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