Purification of antibodies from immune sera
Purified antibodies are often used for direct detection of antigens. In this case the antibody is labeled with an easily identifiable marker for binding and det
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Indirect immunofluorescence of living cells
The surface of living cells retains a more complete antigen or receptor, first use specific mouse-derived monoclonal antibody to bind to the corresponding antig
Hybridization and gene localization experiments in Escherichia coli
The E. coli chromosome is ring-shaped. High-frequency recombinant strains (Hfr) have the F factor integrated into their chromosomes, and the chromosomes of the
Experimental modeling of heterologous in vitro fertilization
Heterozygous in vitro fertilization experimental modeling can be used to: (1) simulate in vivo conditions of male and female gametes from different species of a
Chip Preparation Techniques for Direct Dot Sampling
The direct dispensing method, first developed by Brown Laboratory of Stanford University, involves arranging trace oligonucleotide fragments, cDNA or proteins,
in vitro transcription technology
Usually transcription occurs in the organism, if we will contain RNA transcriptase, NTP and other conditions, in an in vitro cell-free system, using DNA as a te
Hybridoma cloning
Hybridoma cloning is mainly used to (1) clone antibody-positive holes. (2) Separating antibody-secreting cells, antibody-nonsecreting cells, desired antibody (s
Embryo cryopreservation
When embryo freezing is used to cryopreserve living tissues and cells, the freezing process will likely cause damage to the cells.
Novel vector-mediated gene introduction technologies
Currently gene introduction technology is the main technique for the preparation of ips cells, and the improvement of gene vectors promotes the establishment of