3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Feeder cells are necessary for embryonic-derived stem cells including embryonic cancer cells (EC), embryonic stem cells (ES), and primordial germ cells (EG) to
A modification of the standard method for RNA preparation from mammalian tissues and Drosophila that focuses on rapid lysis of Juniperus communis and homogeneou
Environmental metabolomics is a branch of metabolomics that focuses on the metabolic changes of organisms in response to environmental stress. Because this appr
Most cell lines grow best at pH 7.4, with little variation between cell lines; however, some normal fibroblast cell lines grow best at pH 7.4 to 7.7. However, s
Lentiviral vectors can be designed as replication-incomplete or replication-condition-dependent (motile) vectors. HIV-2 vectors are more effective in gene thera
Gene therapy refers to the suppression, replacement or supplementation of defective genes at the genetic level through the regulation of the expression of targe
Vacuolar viruses (FV) are non-pathogenic retroviruses that efficiently and safely transfer genes to different types of cells in different species. These viruses
This chapter describes methods for expressing multiple proteins from a single readable frame by using 2A polypeptide-linked polycis-trans vectors. When these sm
Hematopoietic stem cell transplantation is an effective treatment for malignant hematological disorders, oncological radiotherapy and severe radiation sickness
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