Stem Cell and Gene Therapy
Gene therapy refers to the suppression, replacement or supplementation of defective genes at the genetic level through the regulation of the expression of targe
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Cell and Tissue Targeting
Currently, the life cycle and biology of adenovirus (A d ) infected cells have been well characterized. In A d virus infection and transgene expression, there a
Small RNA virus-based expression vectors
Small R N A viruses comprise a large group of small, pod-less, positive-sense single-stranded R N A viruses. Small R N A viruses have a rapid life cycle that ta
Production and characterization of helper virus-dependent adenoviral vectors
This chapter describes in detail the preparation, amplification and large-scale production of helper virus-dependent adenoviral vectors (H D A d ) for use in ge
Stable virus-producing cell lines for AAV assembly
Cloning of virus-producing cells by infection with a helper virus, stable virus-producing cell lines containing and genes, and r A A V (recombinant adeno-associ
Screening, isolation and characterization of target peptides for ligand-directed gene delivery
Functional vascular mapping of tissue-specific and angiogenesis-related receptors has been more widely promoted and optimized by isolation of receptors, cell li
In vitro packaging of SV40: a pseudoviral gene delivery system
Nuclear extracts from baculovirus-infected insect cells of the meadow moth (Sf9 ) encode the major capsid protein (V P l ) of simian virus 40 (S V 4 0 ). In the
Delivery of genes with HSV/AAV heterozygous amplicon vectors
This chapter describes how to construct H S V /A A V heterotrimeric vectors using an unassisted viral packaging system, and also discusses its advantages and di
Polyomavirus: SV40
This chapter describes how to construct H S V /A A V heterotrimeric vectors using an unassisted viral packaging system, and also discusses its advantages and di