Optimizing in vitro mammalian cell electrotransfection
Electrotransfection can be used for studies of gene function, promoter activity, and gene regulation; it can also be used to create transgenic or knockout embry
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Generation of foamy virus vectors and transduction of hematopoietic stem cells
Foamy virus (F V ), or spumavirus, is a nonpathogenic retrovirus developed as an integrating viral vector. Several potential advantages of F V vectors include a
Generation of VSV-G-pseudotyped retroviral vectors
In order to improve the efficiency of transduction, a strategy called pseudotyping, which combines retroviral particles with other virally encoded capsid protei
Transfection of hippocampal neuronal cells with calcium phosphate co-precipitated plasmid DNA
The use of DNA/phosphorothioate (CAPO 4) co-precipitation to transfect cells is a widely described method. Although this co-precipitation method was proposed mo
Intrauterine microelectroporation for efficient gene transfer in mouse embryos
The introduction of genes into the embryo has been successfully achieved by a new technique of electroporation of the chicken embryo. In contrast, since the mam
Gene transfer into mammalian cells using targeted filamentous phages
Because of their genetic simplicity, phages are commonly used in vector genetic optimization approaches for therapeutic gene transfer. In addition, since phage
Translocation of target genes with surface-modified lentiviral vectors
Two types of surface modifications have been made to lentiviral vectors in order to achieve target gene translocation: (i) taking advantage of the targeting pro
Solid lipid nanoparticles for in vitro mammalian cell transfection
Solid lipid nanoparticles (S L N ) have many technical advantages over gene carriers such as cationic liposomes and cationic polymers. However, in the absence o
Targeted electrically neutral lipid vesicles for systemic delivery of genes
This chapter describes the detailed experimental procedure for encapsulation of nucleic acids by nanolipid particles (N L P ). This N L P carries a polyethylene