Fatty Acid Synthase Mouse mAb - Primary antibody, specific to Fatty Acid Synthase, Mouse IgG2a

Cat. No.: Ab103030

GRADE & PURITY Validated, ExactAb™, 6.25 mg/mL

Application

IF/ICC, IP, WB

Host species

Mouse

Reactive species

Human, Monkey, Mouse, Rat

Isotype

Mouse IgG2a

Clone Number

2F9-B2-B6

Conjugation

Unconjugated

Descripción general

Mouse anti Human Fatty Acid Synthase Antibody, Monoclonal (2F9-B2-B6), could be used for WB, ICC, IF, IP and so on.

Application:

WB: 1/500-1/1000 ICC/IF: 1/50-1/200 IP: 1/20

Protein Function

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

Specifications

Product Name

Fatty Acid Synthase Mouse mAb - Primary antibody, specific to Fatty Acid Synthase, Mouse IgG2a

Sinónimos

[Acyl-carrier-protein] S acetyltransferase antibody | [Acyl-carrier-protein] S malonyltransferase antibody | 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody | 3-oxoacyl-[acyl-carrier-protein] reductase antibody | 3-oxoacyl-[acyl-carrier-pro

Especificaciones y pureza

ExactAb™, Validated, 6.25 mg/mL

Especies hospedadoras

Mouse

Especificidad

Fatty Acid Synthase

Inmunógeno

Purified recombinant human Fatty Acid Synthase protein fragments expressed in E.coli (AA 2400-2511).

Control positivo

WB: HeLa, C6, 3T3, CHO-K1, 293 lysates. ICC/IF: HeLa cells. IP: CHO-K1 lysates.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

6.25 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: Fatty Acid Synthase Mouse mAb (Ab103030)

Fatty Acid Synthase Mouse mAb (Ab103030) - Western Blot
All lanes: Fatty Acid Synthase Mouse mAb (Ab103030) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 273 kDa
Observed band size: 273 kDa
Exposure time: 7.8 s

Western Blot: Fatty Acid Synthase Mouse mAb (Ab103030)

Fatty Acid Synthase Mouse mAb (Ab103030) - Western Blot
All lanes: Fatty Acid Synthase Mouse mAb (Ab103030) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 273 kDa
Observed band size: 273 kDa
Exposure time: 10.0 s

IF/ICC: Fatty Acid Synthase Mouse mAb (Ab103030)

Fatty Acid Synthase Mouse mAb (Ab103030) - IF/ICC
Immunocytochemistry analysis of Fatty Acid Synthase (2F9) in HeLa using Fatty Acid Synthase Mouse mAb (Ab103030).

IP: Fatty Acid Synthase Mouse mAb (Ab103030)

Fatty Acid Synthase Mouse mAb (Ab103030) - IP
Immunoprecipitation analysis of Fatty Acid Synthase (2F9) in CHO-K1 lysates using Fatty Acid Synthase Mouse mAb (Ab103030).

Aplicación

• Aplicación: Dilution info
• IF/ICC:

  1/50-1/200 ;

• IP:

  1/20;

• WB:

  1/500-1/1000 ;

Objetivos asociados (humanos)

FASN Tchem Fatty acid synthase (0 Activities)

Discover Target: FASN

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción