GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactio
Especies hospedadoras
Mouse
Especificidad
FLNA
Inmunógeno
Recombinant fusion protein of human Filamin A.
Control positivo
WB: HeLa, C6, PC3, COS7, NIH3T3 lysates. IF/ICC: Hela cells.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
Filamin A Mouse mAb (Ab103618) - Western Blot All lanes: Filamin A Mouse mAb (Ab103618) at 1/1000 dilution Samples: Lysates at 20 µg per lane
Predicted band size: 280 kDa Observed band size: 280 kDa
Filamin A Mouse mAb (Ab103618) - IF/ICC Immunofluorescent analysis of Filamin A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Filamin A Mouse mAb (Ab103618) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Aplicación
Aplicación
Dilution info
WB
1/1000 - 1/2000
IF/ICC
1/10 - 1/50
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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