GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Especies hospedadoras
Mouse
Especificidad
FLI1
Inmunógeno
A synthetic peptide derived from human FLI1
Control positivo
WB: Jurkat, THP-1 and Ramos cell lysates. IHC: Human hemangioendothelioma and pnet tissues.
Conjugación
Unconjugated
Grado
ExactAb™, Validated
Isotipo
Mouse IgG1
SDS-PAGE
150 kDa
Método de purificación
Immunogen affinity purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
FLI1 Mouse mAb (Ab103739) - Western Blot All lanes: FLI1 Mouse mAb (Ab103739) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 51 kDa Observed band size: 48, 52 kDa FLI1 Mouse mAb (Ab103739) recognizes different isoforms of FLI1. Exposure time: 60.0 s
FLI1 Mouse mAb (Ab103739) - Western Blot All lanes: FLI1 Mouse mAb (Ab103739) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 51 kDa Observed band size: 48, 51 kDa FLI1 Mouse mAb (Ab103739) recognizes different isoforms of FLI1. Exposure time: 100.0 s
FLI1 Mouse mAb (Ab103739) - IHC Immunohistochemical analysis of FLI1 staining in human hemangioendothelioma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FLI1 Mouse mAb (Ab103739) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
FLI1 Mouse mAb (Ab103739) - IHC Immunohistochemical analysis of FLI1 staining in human pnet formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FLI1 Mouse mAb (Ab103739) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Aplicación
Aplicación
Dilution info
WB
1/500-1/1000
IHC
1/100-1/300
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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