IL-6 Mouse mAb - Primary antibody, specific to Human IL-6

Cat. No.: Ab188014

CALIDAD & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Capture Antibody, ELISA

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

C05/5G3

Conjugation

Unconjugated

Descripción general

Mouse anti Human IL-6 Antibody, clone C05/5G3, could be used for a Capture Antibody in sandwich ELISAs

Especificaciones

Product Name

IL-6 Mouse mAb - Primary antibody, specific to Human IL-6

Sinónimos

Interleukin BSF 2 antibody | B cell differentiation factor antibody | B cell stimulatory factor 2 antibody | B-cell stimulatory factor 2 antibody | BSF 2 antibody | BSF-2 antibody | BSF2 antibody | CDF antibody | CTL differentiation factor antibody | Cyto

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induc

Especies hospedadoras

Mouse

Especificidad

Human IL-6

Inmunógeno

Recombinant protein corresponding to Human IL-6

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Pares emparejados

[Ab168996] IL-6 Mouse mAb, Detection Antibody

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Sandwich ELISA - IL-6 Antibody Pair (Ab188014 & Ab168996)

Sandwich ELISA - IL-6 standard curve
Sandwich ELISA detection of Recombinant Human IL-6 Protein (rp156005) using IL-6 Mouse mAb (Ab188014) as capture antibody at concentration of 1.0 µg/mL. Biotinylated IL-6 Mouse mAb (Ab168996) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).

Detection range: 10.0 ng/mL - 10000.0 ng/mL

Indirect ELISA - IL-6 Mouse mAb (Ab188014)

Indirect ELISA - IL-6 standard curve
Immobilized Recombinant Human IL-6 Protein (rp156005) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with IL-6 Mouse mAb (Ab188014). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.

EC₅₀: 30.23 ng/mL

Aplicación

Aplicación

Dilution info

ELISA

1.0 µg/mL as a Capture antibody in ELISA

Objetivos asociados (humanos)

IL6 Tclin Interleukin-6 (0 Activities)

Discover Target: IL6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IL-6 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.