MelanA Mouse mAb - Primary antibody, specific to MelanA, Mouse IgG1

Cat. No.: Ab114904

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

KAA343_6B7

Conjugation

Unconjugated

Descripción general

Mouse anti Human MelanA Antibody, Monoclonal (KAA343_6B7), could be used for WB, IHC, IP and so on.

Application

WB: 1/1000

IHC: 1/200

IP: 1/100

Protein Function

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Specifications

Product Name

MelanA Mouse mAb - Primary antibody, specific to MelanA, Mouse IgG1

Sinónimos

Antigen LB39 AA antibody | Antigen LB39-AA antibody | Antigen SK29 AA antibody | Antigen SK29-AA antibody | MAR1_HUMAN antibody | MART 1 antibody | MART-1 antibody | MART1 antibody | Melan A antibody | Melan A protein antibody | Melanoma antigen recognize

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Especies hospedadoras

Mouse

Especificidad

MelanA

Inmunógeno

Recombinant protein of human MelanA.

Control positivo

WB: SK-MEL-28 lysates. IHC: Human malignant melanoma tissue. IP: SK-MEL-28 cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -80°C,Avoid repeated freezing and thawing

Enviado en

Dry ice packs + Cold packs

Estabilidad y almacenamiento

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: MelanA Mouse Antibody (Ab114904)

MelanA Mouse Antibody (Ab114904) - Western Blot
15 µg of SK-MEL-28 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. MelanA Mouse Antibody (Ab114904) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. MLANA band was visualized using ECL Western Blotting Substrate.
Result: MelanA Mouse Antibody (Ab114904) can detect MLANA by Western blotting.

IP: MelanA Mouse Antibody (Ab114904)

MelanA Mouse Antibody (Ab114904) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of MelanA Mouse Antibody (Ab114904) with SK-MEL-28 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. MelanA Mouse Antibody (Ab114904) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.
Lane 1: SK-MEL-28 lysate
Lane 2: MLANA immunoprecipitated from SK-MEL-28 lysate by MelanA Mouse Antibody (Ab114904)
Lane 3: The same as Lane 2 was IgG isotype control antibody
Result: MelanA Mouse Antibody (Ab114904) can immunoprecipitate MLANA.

IHC: MelanA Mouse Antibody (Ab114904)

MelanA Mouse Antibody (Ab114904) - IHC
IHC-P analysis of malignant melanoma tissue by MelanA Mouse Antibody (Ab114904).IHC-P was performed using sections of the formalin-fixed paraffin-embedded malignant melanoma tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with MelanA Mouse Antibody (Ab114904) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Tumor cells are positively stained at the cytoplasm.

Aplicación

• Aplicación: Dilution info
• WB:

  1/1000 ;

• IP:

  1/100;

• IHC:

  1/200 ;

Objetivos asociados (humanos)

MLANA Tbio Melanoma antigen recognized by T-cells 1 (0 Activities)

Discover Target: MLANA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)