MOB1B Mouse mAb - Primary antibody, specific to MOB1B, Rabbit IgG

Cat. No.: Ab179614

CALIDAD & PUREZA ExactAb™, Validated, See COA

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG2a

Clone Number

OTI3G9

Conjugation

Unconjugated

Descripción general

Mouse anti Human MOB1B Antibody, Monoclonal (OTI3G9), could be used for WB, IHC and so on.

Especificaciones

Product Name

MOB1B Mouse mAb - Primary antibody, specific to MOB1B, Rabbit IgG

Sinónimos

MATS2 | MOB4A | MOBKL1A | MOB1B | MOB kinase activator 1B | MOB1 antibody | Mob1 homolog 1A | Mob1A | Mob1B | Mps one binder kinase activator-like 1A | MATS2 | MOB4A | MOBKL1A | MOB1B | MOB kinase activator 1B | MOB1 antibody

Especificaciones y pureza

ExactAb™, Validated, See COA

Mecanismos bioquímicos y fisiológicos

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and

Especies hospedadoras

Mouse

Especificidad

MOB1B

Inmunógeno

Recombinant full length protein of human MOBKL1A produced in HEK293T.

Control positivo

WB: HEK293T transfected with the pCMV6-ENTRY MOBKL1A cDNA for 48 hrs, HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 lysates. IHC: Human prostate carcinoma, prostate, liver and kidney tissues.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: MOB1B Mouse mAb (Ab179614)

MOB1B Mouse mAb (Ab179614) - Western Blot
All lanes: MOB1B Mouse mAb (Ab179614) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 100.0 s

Western Blot: MOB1B Mouse mAb (Ab179614)

MOB1B Mouse mAb (Ab179614) - Western Blot
All lanes: MOB1B Mouse mAb (Ab179614) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 100.0 s

IHC: MOB1B Mouse mAb (Ab179614)

MOB1B Mouse mAb (Ab179614) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using MOB1B Mouse mAb (Ab179614). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab179614, Dilution 1/50)

IHC: MOB1B Mouse mAb (Ab179614)

MOB1B Mouse mAb (Ab179614) - IHC
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using MOB1B Mouse mAb (Ab179614). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab179614, Dilution 1/50)

IHC: MOB1B Mouse mAb (Ab179614)

MOB1B Mouse mAb (Ab179614) - IHC
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using MOB1B Mouse mAb (Ab179614). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab179614, Dilution 1/50)

IHC: MOB1B Mouse mAb (Ab179614)

MOB1B Mouse mAb (Ab179614) - IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using MOB1B Mouse mAb (Ab179614). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab179614, Dilution 1/50)

Aplicación

Aplicación

Dilution info

WB

1/500-1/1000

IHC

1/50

Objetivos asociados (humanos)

MOB1B Tbio MOB kinase activator 1B (0 Activities)

Discover Target: MOB1B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MOB1B Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.