GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/de
Especies hospedadoras
Mouse
Especificidad
PAK4
Inmunógeno
Recombinant human PAK4 (AA 101-220).
Control positivo
WB: HEK293, Jurkat, HeLa, MCF7, LNCaP, NIH/3T3 and C6 cell lysates. IHC: Human prostate tissue. IP: HEK293 lysates.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, Validated
Isotipo
Mouse IgG1
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A purified
Concentración
1.0 mg/mL
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -80°C,Avoid repeated freezing and thawing
Enviado en
Dry ice packs + Cold packs
Estabilidad y almacenamiento
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
PAK4 Mouse mAb (Ab120121) - Western Blot All lanes: PAK4 Mouse mAb (Ab120121) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 74, 75 kDa PAK4 can function as an antiapoptotic protein (PMID:14517283). And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822). Exposure time: 2.9 s
PAK4 Mouse mAb (Ab120121) - IP Immunoprecipitation was performed by incubation of 2.5 µg of PAK4 Mouse mAb (Ab120121) with HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PAK4 Mouse mAb (Ab120121) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: HEK293 lysate Lane 2: PAK4 immunoprecipitated from HEK293 lysate by PAK4 Mouse mAb (Ab120121) Lane 3: The same as Lane 2 but mouse IgG was used as IgG isotype control antibody Result: PAK4 Mouse mAb (Ab120121) can immunoprecipitate PAK4.
PAK4 Mouse mAb (Ab120121) - IHC IHC analysis of prostate tissue by PAK4 Mouse mAb (Ab120121). IHC was performed using sections of the formalin-fixed paraffin-embedded prostate tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PAK4 Mouse mAb (Ab120121) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Glandular cells are positively stained at the cytoplasm.
Aplicación
Aplicación
Dilution info
WB
1/1000
IP
1/100
IHC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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