PDHA1 Mouse mAb - Primary antibody, specific to PDHA1, Mouse IgG1

Cat. No.: Ab120952

GRADE & PURITY Validated, ExactAb™, 1.14 mg/mL

Application

IF/ICC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

3H2-F8-B5

Conjugation

Unconjugated

Descripción general

Mouse anti Human PDHA1 Antibody, Monoclonal (3H2-F8-B5), could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Specifications

Product Name

PDHA1 Mouse mAb - Primary antibody, specific to PDHA1, Mouse IgG1

Sinónimos

ODPA_HUMAN antibody | PDH antibody | PDHA antibody | PDHA1 antibody | PDHCE1A antibody | PDHE1 A type Iantibody | PDHE1-A type I antibody | PHE1A antibody | Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody | Pyruvate dehydrogenase complex, E1 alpha pol

Especificaciones y pureza

ExactAb™, Validated, 1.14 mg/mL

Especies hospedadoras

Mouse

Especificidad

PDHA1

Inmunógeno

Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli (AA 131-389).

Control positivo

WB: HeLa, HEK293, Hep G2, A549, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

1.14 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: PDHA1 Mouse mAb (Ab120952)

PDHA1 Mouse mAb (Ab120952) - Western Blot
All lanes: PDHA1 Mouse mAb (Ab120952) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 41 kDa
Exposure time: 20.0 s

IF/ICC: PDHA1 Mouse mAb (Ab120952)

PDHA1 Mouse mAb (Ab120952) - IF/ICC
Immunocytochemistry analysis of Pyruvate Dehydrogenase E1 alpha in HeLa using PDHA1 Mouse mAb (Ab120952).

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

Objetivos asociados (humanos)

PDHA1 Tbio Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (0 Activities)

Discover Target: PDHA1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)