CALIDAD & PUREZAKO Validation?Knockout-validated — antibody specificity confirmed using knockout cells/tissue. Use when you need strong evidence the antibody binds only its target.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.See COA
KO Validation, ExactAb™, Validated, See COA ExactAb™,KO Validation,Validated para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.
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Almacenamiento y envío
Store at -20°C,Avoid repeated freezing and thawing Envío Ice chest + Ice pads. Consulte el COA específico del lote para conocer las especificaciones exactas.
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Documentos de calidad
SDS, COA, ficha técnica y hoja de especificaciones disponibles para descargar. COA específico del lote accesible mediante búsqueda por número de lote.
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Prueba documental
Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.
Descripción general
Mouse anti Human RALBP1 Antibody, Monoclonal (OTI2A6), could be used for WB, IHC, IF/ICC, IP and so on.
Especificaciones
Product Name
RALBP1 Mouse mAb - Primary antibody, specific to RALBP1, Rabbit IgG
Sinónimos
RLIP1 antibody | 76 kDa Ral-interacting protein antibody | 76-kDa Ral-interacting protein antibody | Dinitrophenyl S-glutathione ATPase antibody | DNP-SG ATPase antibody | Ral-interacting protein 1 antibody | Ral-interacting protein 1, 76-KD antibody | Ra
Especificaciones y pureza
KO Validation, ExactAb™, Validated, See COA
Mecanismos bioquímicos y fisiológicos
Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of
Especies hospedadoras
Mouse
Especificidad
RALBP1
Inmunógeno
Recombinant f ull length protein of human RALBP1.
Control positivo
WB: HeLa, Hep G2, MCF7, HT-29, NIH/3T3 and C6 cell lysates. IHC: Human breast adenocarcinoma, colon adenocarcinoma, kidney, ovary adenocarcinoma, endometrium adenocarcinoma, prostate, prostate carcinoma tissues. IF/ICC: COS7 cells transiently transfected
Conjugación
Unconjugated
Grado
ExactAb™, KO Validation, Validated
Isotipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificación
Protein G purified
Concentración
See COA
Product Line
Productos biológicos
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
RALBP1 Mouse mAb (Ab124484) - Western Blot All lanes: RALBP1 Mouse mAb (Ab124484) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 76 kDa Observed band size: 91 kDa Exposure time: 60.0 s
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Adenocarcinoma of breast tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Adenocarcinoma of colon tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Kidney tissue within the normal limits using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Adenocarcinoma of ovary tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50)
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Adenocarcinoma of endometrium tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded prostate tissue within the normal limits using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Carcinoma of prostate tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IF/ICC RALBP1 Mouse mAb (Ab124484) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1.
RALBP1 Mouse mAb (Ab124484) - IF/ICC Immunofluorescent staining of Hep G2 cells using RALBP1 Mouse mAb (Ab124484).
RALBP1 Mouse mAb (Ab124484) - KO Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and RALBP1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with RALBP1 Mouse mAb (Ab124484) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Aplicación
Aplicación
Dilution info
WB
1/1000
IF/ICC
1/100
IHC
1/50
IP
4 μg/mL
Documentación
📋 Hoja de datos de seguridad (SDS)
Documento completo sobre peligros, manipulación, almacenamiento y cumplimiento normativo.
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Reseñas
Reseñas de cliente
Preguntas frecuentes
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What does KO Validation mean?
KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RALBP1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
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