Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purified,Lyophilized), CAS No.9026-12-4

CAS: 9026-12-4 Cat. No.: R128612 Peso molecular: 11 kDa Número EC: 232-809-1

GRADE & PURITY EnzymoPure™, ≥2,500 units/mg

Accession #

P00651

Descripción general

Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.
Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

Specifications

Product Name

Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purified,Lyophilized), CAS No.9026-12-4

Sinónimos

RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase

Grado

EnzymoPure™

Especificaciones y pureza

EnzymoPure™, ≥2, 500 units/mg

Mecanismos bioquímicos y fisiológicos

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3’-phosphate group of a guanidine ribonucleotide and 5’-hydroxyl of the adjacent nucleotide. The initial product is a 2

Número de adhesión

P00651

CAS

9026-12-4

Número de la Comisión de Enzimas

3.1.27.3

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

≥2,500 units/mg

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Definición de unidad

One Unit releases the equivalent of one A260 unit of acid-soluble products from yeast RNA in 15 minutes at 37°C, pH 7.5