GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C,Avoid repeated freezing and thawing Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Mouse anti Human RNF21/IFP1 Antibody, Monoclonal (K24023_3C6), could be used for WB, IHC, IP and so on.
Specifications
Product Name
RNF21/IFP1 Mouse mAb - Primary antibody, specific to TRIM34, Mouse IgG2a
Sinónimos
IFP1 antibody | Interferon Responsive Finger Protein 1 antibody | Ring Finger Protein 21 antibody | Ring finger protein 21 interferon responsive antibody | RNF21 antibody | TRIM 34 antibody | TRIM34 antibody | Tripartite motif containing protein 34 antibo
Especificaciones y pureza
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Mecanismos bioquímicos y fisiológicos
Relevance
RNF21 (TRIM34) is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Expression of the TRIM34 gene is up-regulated by interfero
Store at -80°C,Avoid repeated freezing and thawing
Enviado en
Dry ice packs + Cold packs
Estabilidad y almacenamiento
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
RNF21/IFP1 Mouse mAb (Ab125563) - Western Blot All lanes: RNF21/IFP1 Mouse mAb (Ab125563) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 57 kDa Observed band size: 52 kDa Exposure time: 5.0 s
RNF21/IFP1 Mouse mAb (Ab125563) - IHC IHC analysis of placenta tissue by RNF21/IFP1 Mouse mAb (Ab125563). IHC was performed using sections of the formalin-fixed paraffin-embedded placenta tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RNF21/IFP1 Mouse mAb (Ab125563) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Trophoblastic cells are positively stained at the cytoplasm.
RNF21/IFP1 Mouse mAb (Ab125563) - IP Immunoprecipitation was performed by incubation of 2.5 µg of RNF21/IFP1 Mouse mAb (Ab125563) with Jurkat lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. RNF21/IFP1 Mouse mAb (Ab125563) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: Jurkat lysate Lane 2: TRIM34 immunoprecipitated from Jurkat lysate by RNF21/IFP1 Mouse mAb (Ab125563) Lane 3: The isotype control antibody instead of RNF21/IFP1 Mouse mAb (Ab125563) in Jurkat lysate
Result: RNF21/IFP1 Mouse mAb (Ab125563) can immunoprecipitate TRIM34.
Aplicación
Aplicación
Dilution info
IP
1/100
IHC
1/200
WB
1/2000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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