SRPK2 Mouse mAb - Primary antibody, specific to SRPK2, Mouse IgG2a

Cat. No.: Ab129203

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

K29040_13A1

Conjugation

Unconjugated

Descripción general

Anticuerpo monoclonal de ratón contra SRPK2 humana (K29040_13A1), puede utilizarse para WB, IHC, etc.
Aplicación:
WB: 1/1000
IHC: 1/200
Función de la proteína
Cinasa específica de proteínas ricas en serina/arginina que fosforila específicamente a sus sustratos en residuos de serina localizados en regiones ricas en dipéptidos de arginina/serina, conocidas como dominios RS y está implicada en la fosforilación de factores de splicing SR y en la regulación del splicing. Promueve la apoptosis neuronal mediante la regulación al alza de la expresión de ciclina-D1 (CCND1). Esto se consigue mediante la fosforilación de SRSF2, lo que conduce a la supresión de la fosforilación de p53/TP53, aliviando así el efecto represor de p53/TP53 sobre la expresión de ciclina-D1 (CCND1). Fosforila la ACIN1 y la redistribuye de las motas nucleares al nucleoplasma, lo que provoca la regulación al alza de la ciclina A1 pero no de la ciclina A2. Desempeña un papel esencial en la formación del complejo espliceosomal B a través de la fosforilación de DDX23/PRP28. Puede mediar en la fosforilación de la proteína del núcleo del virus de la hepatitis B (VHB). Desempeña un papel negativo en la regulación de la replicación del VHB a través de un mecanismo que no implica la fosforilación de la proteína del núcleo, sino mediante la reducción de la eficiencia de empaquetamiento del ARN pregenómico (ARNpg) sin afectar a la formación de las partículas del núcleo viral.

Specifications

Product Name

SRPK2 Mouse mAb - Primary antibody, specific to SRPK2, Mouse IgG2a

Sinónimos

Anticuerpo serina quinasa SRPK2 | Anticuerpo serina/arginina proteína quinasa específica 2 | Anticuerpo serina/arginina proteína quinasa específica 2 | Anticuerpo serina/treonina proteína quinasa SRPK2 | Anticuerpo SFRS proteína quinasa 2 | Anticuerpo SFR

Especificaciones y pureza

ExactAb™, validado, sin portador, 1,0 mg/mL

Especies hospedadoras

Mouse

Especificidad

SRPK2

Inmunógeno

Recombinant protein of human SRPK2 (AA 321-460)

Control positivo

WB: HeLa and MCF7 cell lysates. IHC: Human cerebellum tissue.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Líquido

Condiciones de almacenamiento de almacenamiento

Conservar a -80°C,Evitar la congelación y descongelación repetidas

Enviado en

Dry ice packs + Cold packs

Estabilidad y almacenamiento

Almacenar a 2-8 ℃ (3 meses). Almacenar a -20 ℃ (12 meses). Almacenar a -80 ℃ (36 meses). Tras la recepción, se recomienda alícuota. Evitar el ciclo de congelación/descongelación.

Imágenes

Western Blot: SRPK2 Mouse mAb (Ab129203)

SRPK2 Mouse mAb (Ab129203) - Western Blot
All lanes: SRPK2 Mouse mAb (Ab129203) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 99 kDa
Exposure time: 100.0 s

IHC: SRPK2 Mouse mAb (Ab129203)

SRPK2 Mouse mAb (Ab129203) - IHC
IHC analysis of cerebellum tissue by SRPK2 Mouse mAb (Ab129203).IHC was performed using sections of the formalin-fixed paraffin-embedded cerebellum tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with SRPK2 Mouse mAb (Ab129203) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Purkinje cells are positively stained at the cytoplasm of cell body and dentrite.

Aplicación

• Aplicación: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/200 ;

Objetivos asociados (humanos)

SRPK2 Tchem SRSF protein kinase 2 (0 Activities)

Discover Target: SRPK2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (SRPK2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.