10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 5 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
Amitriptyline HCl is an inhibitor of bothserotonin transporter (SERT)andnorepinephrine transporter (NET)with Ki of 3.45 nM and 13.3 nM, respectively. Amitriptyline HCl also inhibitshistamine receptor H1,histamine receptor H4,5-HT2andsigma 1 receptorwith K
In vitro
Amitriptyline inhibits Forskolin-stimulated cyclic AMP accumulation with EC50 values of 16.2 μM in intact CHO/DOR cells. Amitriptyline causes a concentration-dependent stimulation of ERK1/2 and GSK-3β phosphorylation with EC50 values of 9.0 μM in CHO/DOR cells. Amitriptyline (15 μM) causes a stimulation of ERK1/2 phosphorylation in C6 cells. Amitriptyline (30 μM) inhibits Forskolin-stimulated adenylyl cyclase activity and antagonizes (−)-U50,488 inhibitory effect in rat nucleus accumbens. Amitriptyline binds the extracellular domain of both TrkA and TrkB and promotes TrkA-TrkB receptor heterodimerization. Amitriptyline (< 500 nM) promotes TrkA autophosphorylation in primary neurons and induces neurite outgrowth in PC12 cells. Amitriptyline selectively protects T17 cells from apoptosis with EC50 of 50 nM. [6]
In vivo
Amitriptyline (15 mg/kg, i.p.) activates TrkA and TrkB receptors and significantly reduces kainic acid-triggered neuronal cell death in mice. Amitriptyline (15 mg/kg and 30 mg/kg, i.p.) dose-dependently decreases the immobility time in the forced swimming test (FST) of mice. Amitriptyline (15 mg/kg, i.p.) shows a significant 24-h rhythm in the immobility time in the forced swimming test (FST) of mice. Amitriptyline (1 mg/kg and 3 mg/kg) significantly increases the total distance travelled of mice in novel cages. Amitriptyline (10 mg/kg p.o., twice daily) considerably attenuates the hypothermic response to 8-OHDPAT and mCPP in mice. Amitriptyline (10 mg/kg p.o., twice daily) significantly reduces serotonin transporter density by approximately 20% in cortex of mice.
Cell Data
cell lines：Mutant cell lines (G401, A204, G402, KYM-1), Wild type cell line (RD, 293, SJCRH30)
Concentrations：
Incubation Time：
Powder Purity:≥99%
| Isomeric SMILES | CN(C)CCC=C1C2=CC=CC=C2CCC3=CC=CC=C31.Cl |
|---|---|
| WGK Germany | 3 |
| RTECS | HO9450000 |
| Alternate CAS | 50-48-6 |
| Molecular Weight | 313.86 |
| Reaxy-Rn | 4164595 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=4164595&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Solubility | Solubility (25°C) In vitro Water: 37 mg/mL (201.01 mM); DMSO: 3 mg/mL (16.29 mM); Ethanol: Insoluble; |
|---|---|
| Melt Point(°C) | 195 °C |
| 1. Cong Lu, Zhen Wei, Yongquan Wang, Shuying Li, Litao Tong, Xinmin Liu, Bei Fan, Fengzhong Wang. (2022) Soy isoflavones alleviate lipopolysaccharide-induced depressive-like behavior by suppressing neuroinflammation, mediating tryptophan metabolism and promoting synaptic plasticity. Food & Function, 13 (18): (9513-9522). [PMID:35993820] [10.1039/D2FO01437H] |
| 2. Cong Lu, Rongjing Gao, Yingyu Zhang, Ning Jiang, Ying Chen, Jing Sun, Qiong Wang, Bei Fan, Xinmin Liu, Fengzhong Wang. (2021) S-equol, a metabolite of dietary soy isoflavones, alleviates lipopolysaccharide-induced depressive-like behavior in mice by inhibiting neuroinflammation and enhancing synaptic plasticity. Food & Function, 12 (13): (5770-5778). [PMID:34038497] [10.1039/D1FO00547B] |
| 3. Libin Wan, Bin Lin, Ruiqin Zhu, Chuixiu Huang, Stig Pedersen-Bjergaard, Xiantao Shen. (2019) Liquid-Phase Microextraction or Electromembrane Extraction?. ANALYTICAL CHEMISTRY, [PMID:31141346] [10.1021/acs.analchem.9b00946] |
| 4. Dan Liu, Ting Qiao, Haiyan Liu, Xiaoling Wang, Zhi-guo Shi. (2017) A simple approach to prepare a sulfone-embedded stationary phase for HPLC. JOURNAL OF SEPARATION SCIENCE, 41 (4): (877-885). [PMID:29205869] [10.1002/jssc.201700751] |
| 5. Qianqian Shang, Yangyang Liu, Menghan Hou, Zhuangzhuang Dong, Stig Pedersen-Bjergaard, Chuixiu Huang, Xiantao Shen. (2025) Automated Formation of Supported Liquid Membranes by Molecular Self-Assembly: A Step Forward for Liquid-Phase Microextraction. ANALYTICAL CHEMISTRY, [PMID:41166073] [10.1021/acs.analchem.5c05134] |
