EnzymoPure™, Concentrated solution of Anstart Taq DNA polymerase, reaction buffer, Mg2+and dNTP premixed EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Product manual
Anstart PCR Mix is a pre-mixed concentrated solution of Anstart Taq DNA polymerase, reaction buffer, Mg2+ (final concentration 2mM) and dNTP. Simply add DNA template and primers to start the PCR reaction. The consistency and efficiency of conventional PCR amplification are optimized.
Product Usage
Hot start method for PCR amplification.
Instructions
1. PCR reaction system settings
a. Dissolve and mix the various solutions required for the PCR reaction, and place them on an ice bath or in an ice box. It is recommended that the reaction PCR liquid be used in aliquots to avoid repeated freezing and thawing.
b. Refer to the following table to set up the PCR reaction. It is recommended to configure the PCR reaction system in an ice bath or on an ice box:
Reagent | Volume | Final concentration |
Anstart PCR Mix（2×） | 25μl |
|
Primer-probe Mix | 3μl | — |
Template | 10μl ※ | — |
Ultra-pure water | Up to 50μl | — |
Total capacity | 50μl | — |
※For different types of templates, the recommended dosage in 50μl reaction volume is as follows:
Mammalian genomic DNA: 0.1-1μg
Escherichia coli genomic DNA: 10-100ng
Plasmid DNA: 0.1-10ng
Too much template DNA can easily lead to non-specific PCR products
c. Use a pipette to mix gently or gently Vortex to mix, and centrifuge for a few seconds at room temperature to allow the liquid to accumulate at the bottom of the tube.
d. Place each set of PCR reaction tubes on the PCR machine to start the PCR reaction.
2. The setting of PCR reaction parameters takes the amplification of 1Kb target fragment as an example
Step | Temperature | Time | Number of cycles |
Predenaturation | 95℃ | 2.5min | 1 |
Transsexual | 94℃ | 30s |
25-35 |
Annealing | 55℃ | 30s | |
Extend | 72℃ | 1min | |
Last extension | 72℃ | 10min | 1 |
a. PCR reaction settings need to be set according to different conditions such as template, primers, PCR product length and GC content, etc.
The reaction conditions include temperature, time and number of cycles.
b. The time setting of STEP4 (extension) needs to be set according to the length of the PCR product, usually the extension time per kb product is 1 min. For example, if the length of the PCR product is 1kb, the extension time can be set to 1min, and the length of the PCR product is 2kb, then the extension time can be set to 2min, and so on.
c. For the initial PCR, in order to ensure that the expected PCR product can be amplified as much as possible, the number of cycles can be set to 35. For semi-quantitative or quantitative PCR reaction cycles, the number of cycles must be properly optimized to make the PCR reaction reach a plateau.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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