GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Host species
Mouse
Specificity
beta actin
Immunogen
Synthetic peptide in beta Actin
Positive Control
IF/ICC: Hela Flow Cytometry: Jurkat
Conjugation
AF488
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Images
beta Actin Mouse mAb (AF488) (Ab219182) - IF/ICC IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (AF488) (Ab219182) at 1/1000 dilution in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
beta Actin Mouse mAb (AF488) (Ab219182) - Flow Cytometry Flow cytometry analysis of beta Actin expression by Jurkat cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with beta Actin Mouse mAb (AF488) (Ab219182) (Red) at 1/1000 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
1:100-1:2000
IF/ICC
1/100-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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