GRADE & PURITYEx:498nm?Fluorescence excitation maximum at 498 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal.Em:517nm?Fluorescence emission maximum at 517 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk.
Ex:498nm, Em:517nm Em:517nm,Ex:498nm for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Casein is considered to be a generic substrate for a broad spectrum of proteases. As native casein this fluoresceinated casein is hydrolyzed by many proteases, and widely used for fluorimetric measurement of protease activity. In the intact substrate, casein is heavily labeled with 5-FITC, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly green fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. We do not recommend that this conjugate be used for fluorescence polarization assay. For fluorescence polarization we can custom-make the lightly labeled fluorescein casein conjugate.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Refer to SDS for further information. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Molecule Type
Protein
Storage and Shipping
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the material to approximately 5 mg/ml by addition of 1mL distilled water. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C, avoid repeated freeze-thaw cycles.
Storage
Store at 2-8°C,Protected from light
Shipped In
Wet ice
Stability And Storage
Product should be stored at 2-8℃ in the dark. After reconstitution, store in aliquots and freeze material at -20℃, avoid freeze/thaw cycle.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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