Collagen IV（human）, CAS No.C390608

CAS: C390608 Cat. No.: C390608

GRADE & PURITY 0.5-5 mg/ml in 0.01mol/L PBS,＞95%

Overview

NCBI accession：NM_001845.4

UniProt accession：P02462

Binding Specificity: Laminin; Nidogen

Human collagen type IV is a native triple helix and heterotrimeric protein containing two α1-like and one α2-like genes. Type 4 collagen forms a sheet-like extracellular matrix in multicellular tissues that exists around certain cell types like adipocytes, skeletal muscle cells, heart muscle cells, and smooth muscle cells. Human Collagen IV is extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment. The Collagen type 4 was purified by using differential salt precipitation. Purity and retention of the native helical structure were controlled by SDS-PAGE and by reaction with anti-collagen type-specific monoclonal antibodies.

Physical form

Liquid purified protein.

Specifications

Product Name

Collagen IV（human）, CAS No.C390608

Specifications & Purity

0.5-5 mg/ml in 0.01mol/L PBS, ＞95%

Biochemical and Physiological Mechanisms

Human collagen type IV is associated with Alport and Goodpasture′s syndrome, as well as several rheumatological and dermatological diseases including, acquired epidermolysis bullosa.

Predicted molecular weight

300 kDa

CAS

C390608

Molecule Type

Protein

Storage and Shipping

Concentration

0.5-5 mg/ml in 0.01mol/L PBS,＞95%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

References

1. Fanyan Chen, Bingkuan Zhu, Yu Fang, Zilüe Li, Zhigang Lei, Zechao Xue, Tao Shen, Sha Zhou, Xiaojun Chen, Lei Xu, Yalin Li, Jifeng Zhu, Wei Hu, Chuan Su.  (2025)  Schistosoma japonicum leishmanolysin SjLLPi1 facilitates the invasion of cercariae into the host skin.  PLoS Pathogens,  21  (8): (e1013446).  [PMID:40857348] [10.1371/journal.ppat.1013446]