GRADE & PURITY 10mM in DMSO

Synonyms

Doxycycline hydrochloride hemiethanolate hemihydrate | 2-Naphthacenecarboxamide, 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,5,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-, hydrochloride, (4S,4aR,5S,5aR,6R,12aS)-, compd. with ethanol, hydrate (2:2:1

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

Doxycycline Hyclate (WC2031) Doxycycline Hyclate (WC2031, Doxycycline hydrochloride hemiethanolate hemihydrate) is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix m
In vitro

Doxycycline hyclate 10% (DOXY) has significant toxicity effects on osteoblast cells compared to the control group with a mean cell viability of 84%. Doxycycline hyclate 10% (DOXY) results in a significantly higher alkaline phosphatase activity (approximately 56%) than the control in osteoblasts. Doxycycline hyclate 10% (DOXY) seems to slightly altered the morphology of the osteoblasts.

In vivo

Doxycycline hyclate is an effective treatment for acute E. canis infection in dogs. Doxycycline hyclate (5 mg/kg, orally) decreases the collagenase activity in corneas obtained from rabbits. Doxycycline hyclate (5 mg/kg, orally) promotes corneal reepithelialization in the rabbit alkali-burn model, a result, perhaps, of the drug\'s ability to inhibit excessive collagenase activity. Doxycycline hyclate results in 43% of prophylaxis in orally treated mice, in contrast, the sustained-release doxycycline hyclate completely protects mice from infection and resultant pathology. Doxycycline hyclate results in bioavailability of 70%, Cmin of 1 mg/L, Cmax of 2.3 mg/L and elimination half-life of 7 hours in calves with immature rumen function.
Cell Data

cell lines：

Concentrations：

Incubation Time：

Powder Purity:≥95%

Specifications

Synonyms

Doxycycline hydrochloride hemiethanolate hemihydrate | 2-Naphthacenecarboxamide, 4-(dimethylamino)-1, 4, 4a, 5, 5a, 6, 11, 12a-octahydro-3, 5, 10, 12, 12a-pentahydroxy-6-methyl-1, 11-dioxo-, hydrochloride, (4S, 4aR, 5S, 5aR, 6R, 12aS)-, compd. with ethanol, hydrate (2:2:1

Specifications & Purity

10mM in DMSO

Biochemical and Physiological Mechanisms

Doxycycline Hyclate (WC2031, Doxycycline hydrochloride hemiethanolate hemihydrate) is a member of the tetracycline antibiotics group, and is commonly used to treat a variety of infections. It is also an inhibitor of matrix metallo-proteinases (MMP).

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Action Type

INHIBITOR

Names and Identifiers

• Isomeric SMILES: CCO.C[C@@H]1[C@H]2[C@@H]([C@H]3[C@@H](C(=O)C(=C([C@]3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.C[C@@H]1[C@H]2[C@@H]([C@H]3[C@@H](C(=O)C(=C([C@]3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.O.Cl.Cl
• WGK Germany: 3
• RTECS: QI8925000
• Alternate CAS: 10592-13-9
• PubChem CID: 54686183
• Molecular Weight: 512.94
• Beilstein: 5702728

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro
• Specific Rotation[α]: -104° (C=1,0.01mol/L HCl(MeOH))

References

1. Zhen-Wen Zhang, Ao-Jun Qiu, Geng-Jin-Sheng Cheng, Yu Fan, Yu Xiao, Yan-Zhu Liu, Qian-Yong Cao.  (2025)  A novel Eu(III)-based nano-aggregation for ratiometric fluorescence sensing of tetracycline.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.114507]

2. Zhao Tengzhi, Wang Zhaofeng, Zhang Runke, Xu Hui, Wang Hua, Yu Ying.  (2025)  Carrot-Derived Nitrogen-Doped Dual-Emission Carbon Dots for Ratiometric Detection of Tetracycline and Oxytetracycline.  JOURNAL OF FLUORESCENCE,      [PMID:40924358] [10.1007/s10895-025-04531-4]

3. Guanhua Liu, Luting Wang, Anan Zhao, Leijie Guo, Liya Zhou, Ying He, Li Ma, Yunting Liu, Jing Gao, Yanjun Jiang.  (2023)  Biocatalytic membranes with crosslinked enzyme aggregates for micropollutant removal.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2023.147635]

4. Fuhua Wei, Meng Nie, Qinhui Ren, Xiang Yu, Hua Li, Hongliang Chen, Mengting He, Zhao Liang, Siyuan Wang, Dongxue Han.  (2023)  Preparation of bimetallic metal–organic frameworks for adsorbing doxycycline hydrochloride from wastewater.  APPLIED ORGANOMETALLIC CHEMISTRY,  37  (9): (e7212).  [PMID:] [10.1002/aoc.7212]

5. Dong Wang, Yumeng Wang, Luheng Jing, Lei Wang, Tonghui Zhang, Xuefen Wang.  (2023)  L-phenylalanine-assisted construction of hierarchically structured Fe(OH)3@Co2(OH)2CO3 with enhanced catalytic activities for the degradation of persistent organic pollutants.  Journal of Cleaner Production,      [PMID:] [10.1016/j.jclepro.2023.138220]

6. Yating Wang, Chaosheng Peng, Airu Liu, Jinyue Song, Xingang Li, Tao Jiang.  (2022)  Novel intramolecular-tailored g-C3N4 with accelerated charge delivery for photocatalytic tetracycline degradation and hydrogen production: experimental studies and theoretical analyses.  Journal of Materials Chemistry C,  11  (3): (1165-1172).  [PMID:] [10.1039/D2TC04350E]

7. Cheng He, Jiaxin Ma, Huan Xu, Changzi Ge, Ziru Lian.  (2022)  Selective capture and determination of doxycycline in marine sediments by using magnetic imprinting dispersive solid-phase extraction coupled with high performance liquid chromatography.  MARINE POLLUTION BULLETIN,      [PMID:36307947] [10.1016/j.marpolbul.2022.114215]

8. Dong Wang, Yumeng Wang, Xuefen Wang.  (2022)  Heterostructured ZIF-67@α-MnO2 based nanofibrous membranes for highly effective removal of low-molecular-weight pollutants from wastewater.  SEPARATION AND PURIFICATION TECHNOLOGY,      [PMID:] [10.1016/j.seppur.2022.122225]

9. Yan-Mei Liang, Hui Yang, Bo Zhou, Yang Chen, Min Yang, Ke-Su Wei, Xiu-Fang Yan, Chao Kang.  (2021)  Waste tobacco leaves derived carbon dots for tetracycline detection: Improving quantitative accuracy with the aid of chemometric model.  ANALYTICA CHIMICA ACTA,      [PMID:35033278] [10.1016/j.aca.2021.339269]

10. Yijian Zhao, Qinzhi Wang, Haihua Wang, Hui Zhangsun, Xinyu Sun, Tong Bu, Yingnan Liu, Wenze Wang, Zhihao Xu, Li Wang.  (2021)  Europium-based metal-organic framework containing characteristic metal chains: A novel turn-on fluorescence sensor for simultaneous high-performance detection and removal of tetracycline.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2021.129610]

11. Yanmin Shen, Wenju Liu, Chen Sun, Paifeng Shi.  (2020)  Solubility Measurement and Correlation of Doxycycline Hyclate in 10 Pure Solvents and Mixtures Solvents at 278.15–323.15 K.  JOURNAL OF CHEMICAL AND ENGINEERING DATA,      [PMID:] [10.1021/acs.jced.0c00097]

12. Junjian Chen, Xuetao Shi, Ye Zhu, Yunhua Chen, Meng Gao, Huichang Gao, Lei Liu, Lin Wang, Chuanbin Mao, Yingjun Wang.  (2020)  On-demand storage and release of antimicrobial peptides using Pandora's box-like nanotubes gated with a bacterial infection-responsive polymer.  Theranostics,      [PMID:31903109] [10.7150/thno.38388]

13. Yanmin Shen, Wenju Liu, Zehua Bao.  (2019)  Solubility measurement and thermodynamic modeling of Doxycycline Hyclate two binary solvent systems at 276.95–323.75 K.  JOURNAL OF MOLECULAR LIQUIDS,      [PMID:] [10.1016/j.molliq.2019.111138]

14. Yan Zhao, Lianfang Chen, Fenglin Tang, Lilei Zhang, Qiang Yang, Xiupei Yang.  (2025)  Boosting peroxymonosulfate activation for complete removal of gatifloxacin by a bead-chain zeolitic imidazolate framework composite.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:39837247] [10.1016/j.jcis.2025.01.102]

15. Liting Yang, Zhaoxiao Hao, Yue Yin, Lijian Du, Xiangyu Liu, Huan Zhang, Jinfang Wu, Fang Guo, Jian Liu, Wenbo Wang.  (2025)  Coadsorbent boosts Caragana-derived carbon for breakthrough antibiotic capture in a robust nanosheet-on-carbon architecture.  Journal of Environmental Chemical Engineering,      [PMID:] [10.1016/j.jece.2025.116426]

16. Yue Zhou, Yuanyuan Jiang, Xiangyu Chen, Hongchen Long, Mao Zhang, Zili Tang, Yufang He, Lei Zhang, Tao Le.  (2024)  Enhanced Sensitivity and Accuracy of Tb3+-Functionalized Zirconium-Based Bimetallic MOF for Visual Detection of Malachite Green in Fish.  Foods,  13  (17): (2855).  [PMID:39272620] [10.3390/foods13172855]

17. Zhaofeng Wang, Hui Xu, Lihua Wang, Xinxiang Zheng, Tengzhi Zhao, Bowen Qu, Hua Wang.  (2025)  Highly-sensitive ratiometric determination of tetracyclines and smartphone-assisted visual assay by carrot-derived nitrogen-doped carbon dots-europium ion dual-emission fluorescence probe.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.113398]

18. Yuchi Zhang, Le Han, Bohan Li, Yan Xu.  (2024)  High-performance ratiometric fluorescence detection and removal of tetracycline in milk based on CDs@ZSM-5:Eu3+.  FOOD CHEMISTRY,      [PMID:39340904] [10.1016/j.foodchem.2024.141441]

19. Hongchao Dai, Lei Yuan, Luyao Fan, Jia Yang, Xinan Jiao.  (2024)  Occurrence and risk-related features of Bacillus cereus in fluid milk.  INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY,      [PMID:] [10.1111/1471-0307.13054]

20. Tiantian Wang, Lingfeng Zhao, Guanhua Liu, Xiaoyang Yue, Xiaobing Zheng, Li Ma, Yunting Liu, Mimi Chen, Yanjun Jiang.  (2025)  Preparation of SCOF/UiO-66-NH2 immobilized laccase biocatalytic membrane for micropollutants removal from water.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.161310]

21. He Ying, Li Wei, Zhang Meiling, Wang Hui, Lin Peilu, Yu Ying, Huang Bin, Hao Meng, He Jianuo, Kong Weiyao, Luo Dan, Xu Tengteng, Wang Jiaqi, Huang Ying, Zhao Qinwen, Liu Ying, Zhang Jie, Nian Yong, Zhang Lei, Zhu Bo, Yin Chengqian.  (2025)  PTPN23-dependent activation of PI3KC2α is a therapeutic vulnerability of BRAF-mutant cancers.  JOURNAL OF EXPERIMENTAL MEDICINE,  222  (3): (e20241147).  [PMID:39841180] [10.1084/jem.20241147]

22. Dong Wang, Siping Ding, Zhenzhen He, Tonghui Zhang, Xuefen Wang.  (2024)  Tailor-made Co3O4@CeO2 based nanofibrous membrane with enhanced catalytic reactivity for efficient degradation of antibiotic.  COMPOSITES PART B-ENGINEERING,      [PMID:] [10.1016/j.compositesb.2024.111424]

23. Yue Jin, Yi Chen, Dayu Zhou, Li Wu, Haolan Zhang, Jinchuan Gu.  (2025)  One-step hydrothermal synthesis of lithium slag-based zeolite for the ultrafast photocatalytic degradation of tetracycline.  JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,      [PMID:] [10.1016/j.jphotochem.2025.116783]

24. Dian Li, Yun Chen, Shangbin Kao, Canlong Yan, Tianyu Zheng, Yuan Qin, Xiumei Zhou, Xiang Shao, Biao Huang.  (2025)  Establishment and Application of a Method for Detecting Tetracycline Antibiotics in Milk Based on AlphaLICA Technology.  Analytical Methods,      [PMID:41493018] [10.1039/D5AY01720C]

25. Zhike Wang, Yanan Chen, Cunling Ye.  (2025)  Transforming β-cyclodextrin into a non-conventional luminophore via H2O2 oxidation and its application in detection of tetracyclines.  Journal of the Taiwan Institute of Chemical Engineers,      [PMID:] [10.1016/j.jtice.2025.106437]

26. Huang Junshi, Huang Bolin, Huang Shuanggen, Wang Xiaobin, Zhao Jinhui.  (2026)  Design and predictive modeling of a veterinary drug detection sensor in paddy field water based on artificial neural networks.  Scientific Reports,      [PMID:41688517] [10.1038/s41598-026-38752-9]