Elastase from porcine pancreas(Lyophilized), CAS No.39445-21-1

CAS: 39445-21-1 Cat. No.: E128657 EC Number: 254-453-6 PubChem CID: 363902459

GRADE & PURITY EnzymoPure™, ≥3 units/mg protein

Accession #

P00772

Overview

Product Describtion:

Porcine pancreatic elastase has a molecular weight of 25.9 kDa, and a pH optimum of 8.5. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Soybean trypsin inhibitor and kallikrein inhibitor suppress proteolytic but not elastolytic activity. Elastase is assayed using a method adapted from that of Feinstein et al., Biochem. Biophys. Res. Comm., 50, 1020 (1973) and using the more soluble substrate of Bieth et al., Biochem. Med., 11, 350 (1974).The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells.

Specifications

Product Name

Elastase from porcine pancreas(Lyophilized), CAS No.39445-21-1

Synonyms

Elastase from hog pancreas | Pancreatopeptidase E | pancreatic elastase | pancreatic elastase I | Elastase, Porcine pancreas | CELA1 | ELA1 | ELS1 | Elastase-1

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥3 units/mg protein

Biochemical and Physiological Mechanisms

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Accession #

P00772

CAS

39445-21-1

Enzyme Commission Number

EC: 3.4.21.36

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥3 units/mg protein

Storage

Store at 2-8°C

Shipped In

Wet ice

Unit definition

One Unit cleaves one micromole of N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroanilide per minute at 25°C, pH 8.0.

References

1. Tao Li, Yongtao Yan, Linlin Han, Mingkun Li, Shiyu Liu, XinYang Zhou, Jungjoon Lee, Xiaomin Li, Yuqing Zhao.  (2024)  Ginseng fruit rare saponins (GFRS), a promising anti-wrinkle agent: Evidence of its antioxidant effect and its capacity to prevent matrix metalloproteinase (MMPs) expression in vitro and in vivo.  INDUSTRIAL CROPS AND PRODUCTS,      [PMID:] [10.1016/j.indcrop.2024.118716]