Exonuclease III

Cat. No.: rp216608

GRADE & PURITY Suitable for molecular biology, BioReagent, PCR Reagent, for DNA synthesis, EnzymoPure™, For In Vitro Transcription, for DNA and RNA applications, 100 U/μl

Overview

Storage buffer: 50mM Tris, 50mM KCl, 1mM DTT, 0.05mM EDTA, 50% Glycerol, 200 μg/ml HSA, pH 8.0

Exonuclease III has 3 '→5' exonuclease activity on double-stranded DNA, which can degrade flat end, 3 'end dented and incised DNA, and degrade DNA molecules from the 3' end. For 3 'protruding ends, especially protruding DNA molecules 4 nt or longer cannot be cut at all. In addition, the active site of the enzyme depends on the helical structure and varies according to the sequence (C>A=T>G).

Product composition:

rp216608	Component	5KU	25KU	Storage
rp216608A	Exonuclease III	50 μL	250 μL	-20°C. Avoid freeze/thaw cycle
rp216608B	10×Exonuclease III Reaction Buffer	750 μL	3.75 mL	-20°C. Avoid freeze/thaw cycle

Source
E.coli

Enzyme Activity Definition
The amount of enzyme required to catalyze the production of 1 nmol acid-soluble total nucleotide in a 50 μl reaction system at 37℃ for 30 min is defined as 1 unit (U).

Applications

(1) Non-directional nested deletion;
(2) Site-directed mutagenesis;
(3) Preparation of chain specific probes;
(4) Preparation of single-stranded substrates for dideoxy sequencing.
Protocol
(1) Configure the reaction system, as shown in the following table

Component	Volume
DNA	5 μg
10×Exonuclease III Reaction Buffer	5 μl
Exonuclease III	0.5 μl
Nuclease-free Water	Up to 50 μl

(2) Incubation at 37 "C for 30min.
(3) Incubation at 75°C for 10 min terminated the reaction.
Cautions
(1) The reaction temperature, the concentration of salt ions in the system and the ratio of enzyme to DNA will affect the activity of the enzyme.
(2) This product is for scientific research only and shall not be used for other purposes.

Specifications

Product Name

Exonuclease III

Synonyms

Exonuclease III

Grade

BioReagent, for DNA and RNA applications, for DNA synthesis, For In Vitro Transcription, PCR Reagent, Suitable for molecular biology, EnzymoPure™

Specifications & Purity

BioReagent, PCR Reagent, for DNA synthesis, Suitable for molecular biology, EnzymoPure™, For In Vitro Transcription, for DNA and RNA applications, 100 U/μl

Molecule Type

Enzyme

Storage and Shipping

Concentration

100 U/μl

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.