Can cell lysate lysed cells be followed by immunoprecipitation?
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Can cell lysate lysed cells be followed by immunoprecipitation?
·Reviewed·Published ·Updated ·8 min read
Can cell lysate lysed cells be followed by immunoprecipitation?
No. Cell lysate for immunoprecipitation is a lysate that lyses cells under non-denaturing conditions. Conventional RIPA lyses contain SDS, which can denature proteins and affect protein interactions. Lysates that do not contain denaturing agents are recommended for immunoprecipitation.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Aladdin Scientific. "Can cell lysate lysed cells be followed by immunoprecipitation?" Aladdin Knowledge Base, updated Aug 26, 2024. https://www.aladdinsci.com/us_en/faqs/can-cell-lysate-lysed-cells-be-followed-by-immunoprecipitation-en.html
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