Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Protein carbonyls are among the most commonly used and most stable endpoint markers of oxidative protein damage. Compared with transient free radicals or short-lived oxidative intermediates, protein carbonyls are more suitable for quantitative comparison of oxidative injury in cells, tissues, ...
Amino acid analysis is a fundamental technical component in biological sample analysis, nutritional evaluation, metabolism research, and fermentation process control. Its analytical targets include free amino acids, total amino acids after hydrolysis, specific single amino acids, and certain ...
In bottom-up proteomics, the digestion step determines peptide-length distribution, accessibility of cleavage sites, peptide physicochemical properties, and subsequent mass-spectrometric detectability. It is therefore a key upstream determinant of proteome coverage depth and quantitative ...
Carboxypeptidases are a class of exopeptidases that act on amino acid residues at the C-terminus of peptides or proteins, and they have important value in protein processing, bioactive peptide maturation, terminal structure trimming, and experimental analysis.
Immunoprecipitation (IP) and co-immunoprecipitation (Co-IP) are two foundational approaches in protein research used for selective enrichment of a target protein and for validating protein–protein interactions, respectively. Immunomagnetic beads immobilized with antibodies or nanobodies ...
The blue agave (Agave tequilana Weber var. Azul), a plant indigenous to the Jalisco region in Mexico, is a significant economic product. Legally, it is the sole plant permitted for use in tequila production. Tequila is crafted from the fermented and distilled juices of A. tequilana. On a global ...
GC-IRMS: Differentiating natural and synthetic sources of menthol by carbon and hydrogen isotope fingerprints
In biological sample analysis, the presence of proteins often interferes with small molecule detection, and some enzyme activities may affect the analysis results. Perchloric acid (PCA) precipitation has become a widely used method due to its ability to efficiently remove proteins and its ...
Illustrate how carbon isotope fingerprints identify adulteration of commercially available coconut juice.
Is the gel stained with this stain compatible with in-gel digestion and mass spectrometry?
Techniques often explored alongside immunological experiments.
