Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
β-Glucan is a class of polysaccharides formed by glucose residues linked through β-glycosidic bonds. It is widely present in oats, barley, yeast, fungi, algae and some microbial-derived materials. β-Glucans from different sources vary greatly in linkage type, branching degree, molecular ...
Protein carbonyls are among the most commonly used and most stable endpoint markers of oxidative protein damage. Compared with transient free radicals or short-lived oxidative intermediates, protein carbonyls are more suitable for quantitative comparison of oxidative injury in cells, tissues, ...
Vitamin C content determination is not merely a matter of quantification, but rather a comprehensive analytical issue involving definition of the target analyte form, sample stabilization procedures, methodological selection, and the interpretive boundaries of analytical results. Because vitamin ...
Pancreatin is a composite enzyme preparation derived from animal pancreas and typically contains proteolytic activity (often represented by trypsin activity), pancreatic amylase activity, and pancreatic lipase activity. A defining feature is its synergistic hydrolytic capacity toward protein, ...
Double-stranded deoxyribonucleases (double-stranded deoxyribonucleases, dsDNases) are nucleases that use double-stranded DNA (dsDNA) as substrates, catalyze phosphodiester-bond hydrolysis, and degrade long DNA polymers into oligonucleotides or mononucleotides.
Techniques often explored alongside immunological experiments.
