Furin, CAS No.141760-45-4

CAS: 141760-45-4 Cat. No.: F755284

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, expressed in Baculovirus-Sf9 Cells,2,000 U/ml

Accession #

P09958

Expression system

Baculovirus-Sf9 Cells

Bioactivity

2,000 U/ml

Overview

Furin is a ubiquitous subtilisin-like proprotein convertase. It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network. Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor. The minimal cleavage site is Arg-X-X-Arg'. However, the enzyme prefers the site Arg-X-(Lys/Arg)-Arg'. An additional arginine at the P6 position appears to enhance cleavage. Furin is inhibited by EGTA, α1- Antitrypsin Portland and polyarginine compounds. 

Source: Isolated from Spodoptera frugiperda (Sf9) cells infected with recombinant baculovirus carrying truncated human furin.

Assay Conditions for Unit Activity

Two fold dilutions of Furin are incubated with 25 µg MBP-FN-paramyosin-ΔSal substrate in 20 mM HEPES, 0.1% Triton X-100, 1 mM CaCl2, 0.2mM β-mercaptoenthanol (pH 7.5 @ 25°C) in a 25 µl reaction. The reaction mix is incubated at 25°C for 6 hours. Separation of reaction products are visualized by SDS-PAGE.

Specifications

Product Name

Furin, CAS No.141760-45-4

Synonyms

dibasic processing enzyme | paired basic amino acid residue cleaving enzyme | prohormone convertase | FURIN | FUR | PACE | SPC1 | PCSK3

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, expressed in Baculovirus-Sf9 Cells, 2, 000 U/ml

Biochemical and Physiological Mechanisms

Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif. Mediates processing of TGFB1, an essential step in TGF-beta-1 activation. Converts through proteolytic cleavage the non-functional Brain na

Bioactivity

2, 000 U/ml

Accession #

P09958

Predicted molecular weight

52.7 kDa

Note

Both Furin and Onchocerca volvulus Blisterase will cleave peptide substrates with the sequence, Arg-X- (Lys/Arg)-Arg. However, the ability of either enzyme to cleave a particular protein substrate depends on its tertiary structure as well as on the amino acids immediately surrounding the cleavage site.

CAS

141760-45-4

Enzyme Commission Number

3.4.21.75

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in Baculovirus-Sf9 Cells,2,000 U/ml

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-FN-paramyosin-ΔSal substrate to 95% completion in 6 hours at 25°C in a total reaction volume of 25 µl.