β-Glucuronidase from Helix pomatia, CAS No.9001-45-0

CAS: 9001-45-0 Cat. No.: G755243 EC Number: 232-606-8

GRADE & PURITY Type H-3, aqueous solution,≥90,000 units/mL

Accession #

P09811

Overview

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application:

β-Glucuronidase from Helix pomatia has been used:

• to convert isoflavone conjugates to their aglycone forms in urine and soymilk samples for high performance liquid chromatography (HPLC) detection
• as a component in the protoplasting solution
• for the generation of aromatic and phenolic compounds from the plasma and urine samples for gas chromatography-mass spectrometry (GC-MS)
• to hydrolyze glucuronide and sulfate conjugated metabolites in fecal samples

β-glucuronidase was used in the measurement of aromatization by a urine technique suitable for the evaluation of aromatase inhibitors in vivo.

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Specifications

Product Name

β-Glucuronidase from Helix pomatia, CAS No.9001-45-0

Specifications & Purity

Type H-3, aqueous solution, ≥90, 000 units/mL

Biochemical and Physiological Mechanisms

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.Used for the hydrolysis of glucuronide co

Accession #

P09811

CAS

9001-45-0

Enzyme Commission Number

3.2.1.31(BRENDA, IUBMB)

Molecule Type

Enzyme

Storage and Shipping

Concentration

Type H-3, aqueous solution,≥90,000 units/mL

Storage

Store at 2-8°C

Shipped In

Wet ice

References

1. Bairen Du, Jingjing Zhang, Yanjie Dong, Junwei Wang, Longwen Lei, Rengfei Shi.  (2020)  Determination of testosterone/epitestosterone concentration ratio in human urine by capillary electrophoresis.  STEROIDS,      [PMID:32603755] [10.1016/j.steroids.2020.108691]

2. Di Wu, Na Hu, Jichao Liu, Guangsen Fan, Xiuting Li, Jing Sun, Chunji Dai, Yourui Suo, Guoliang Li, Yongning Wu.  (2018)  Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection.  TALANTA,      [PMID:30172485] [10.1016/j.talanta.2018.07.073]