Goat Anti-Human IgG (AF660) - Secondary antibody

Cat. No.: Ab217304

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Goat

Reactive species

Human

Isotype

Goat IgG

Conjugation

AF660

Overview

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are suitable for IF/ICC and Flow Cytometry.

Specifications

Product Name

Goat Anti-Human IgG (AF660) - Secondary antibody

Synonyms

Goat Anti-Human IgG H&L Antibody | Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human I

Specifications & Purity

ExactAb™, Validated, 1.0 mg/mL

Host species

Goat

Conjugation

AF660

Excitation (nm)

Red Laser(633nm)

Emission(nm)

691nm

Ex/Em(nm)

Ex:663nm;Em:691nm

Grade

ExactAb™, Validated

Purification Method

antigen affinity chromatography

Concentration

1.0 mg/mL

Storage and Shipping

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF/ICC: Goat Anti-Human IgG (AF660) (Ab217304)

Goat Anti-Human IgG (AF660) (Ab217304) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (AF660) (Ab217304) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Human IgG (AF660) (Ab217304)

Goat Anti-Human IgG (AF660) (Ab217304) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Goat Anti-Human IgG (AF660) (Ab217304) at a dilution of 1/900 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  1/100-1/1000 ;

• IF/ICC:

  1/100-1/1000 ;