GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
Host species
Goat
Specificity
Rabbit IgG
Immunogen
Rabbit IgG
Conjugation
AF594
Excitation (nm)
Yellow-Green Laser(594nm)
Emission(nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
Grade
ExactAb™, Validated
Purification Method
Antigen affinity purified
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
Images
Goat Anti-Rabbit IgG H&L (AF594) (Ab181957) - IF/ICC IF analysis of HA tag (Red) in Hela cells transfected with a eGFP-HA tag. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant HA tag Antibody (Ab106771) at 0.5 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Rabbit IgG H&L (AF594) (Ab181957) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Rabbit IgG H&L (AF594) (Ab181957) - Flow Cytometry Flow Cytometry analysis of HA tag expression by HEK293T cells transfected with a eGFP-HA tag (red) compared to an isotype control (blue). The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes and then stained with Recombinant HA tag Antibody (Ab106771) at 0.5 μg/mL for 30 minutes at 4°C in the dark. Then incubated with Goat Anti-Rabbit IgG H&L (AF594) (Ab181957) at a dilution of 1/900 for 1 hour at room temperature in the dark. Blue - Rabbit IgG (Ab170215). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
1/100-1/1000
IF/ICC
1/100-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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