Human TDP-1

Cat. No.: H489668

Overview

Description

Aladdin provides purified Human Tyrosyl DNA Phosphodiesterase (Tdp1) purified to homogeneity to a single 68 kDa band on SDS-PAGE.  Tdp1 is catalytically active and will remove a terminal 3’ phosphotyrosyl group from a specifically designed oligo target site (oligo-Tyr).  Additionally, Aladdin scientists have demonstrated that Tdp1 will release topo I covalently attached to genomic DNA isolated from cells treated with camptothecin using Aladdin’s In Vivo Link Kit.  Tdp1 is thought to be important in repair of topo/DNA adducts that form following topo-active drug therapy; therefore, there is considerable interest in identifying drugs that target Tdp1.  Such drugs may work well in combination with topo targeting strategies.

Figure 1.  SDS-PAGE of Aladdin Purified Tdp1.  The gel was intentionally overloaded with 20ug of protein to reveal minor breakdown products.

Droduction info

Unit Defintion:

One unit of Tdp1 can release the terminal Tyrosine from 20 ng of substrate (oligo-Tyr) in 30 min at 37° C.

Tdp1 Reaction:

Release of topo I from the covalent complex is shown below.

Tdp1 Assays:

Biochemical assays for Tdp1 can be carried out using an oligo nucleotide with a 3’ terminal tyrosine residue.  Release of tyrosine from a 5’ 32P end labeled oligo is easily measured using a denaturing polyacrylamide gel or DNA sequencing gels. 

Shipping&storage

This product is shipped on wet ice and should be stored at – 20°C.

Specifications

Product Name

Human TDP-1

Molecule Type

Peptide

Storage and Shipping

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads