10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 24 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
Nocodazole (R17934, Oncodazole, NSC238159) is a rapidly-reversible inhibitor of microtubule polymerization, also inhibitsAbl,Abl(E255K)andAbl(T315I)withIC50of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole inducesapoptosis.
In vitro
Nocodazole is a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK with Kd of 0.091 μM, 1.6 μM, 1.8 μM and 1.6 μM, respectively. In addition, the Kd of Nocodazole for Abl(E255K) phosphorylated, Abl(T315I) phosphorylated, BRAF(V600E) and PI3Kγ is 0.12 μM, 0.17 μM, 1.1 μM and 1.5 μM, respectively. Nocodazole induces apoptosis in chronic lymphocytic leukemia cells. Nocodazole inhibits insulin-stimulated glucose transport. Nocodazole decreases apoptosis in some human colon carcinoma cells. Nocodazole impairs the morphology and directionality of migrating medial gan-glionic eminence cells. At high concentrations, Nocodazole rapidly depolymerizes microtubules in cells, while low concentrations of Nocodazole inhibit microtubule dynamic instability. Mitotic cells incubated with different concentrations of Paclitaxel are inhibited from progressing to G1 phase 6 hours after release from the Nocodazole block, with a median inhibitory concentration of 4 nM. Nocodazole-pretreated cells exposed to Paclitaxel in the absence of Nocodazole only form free-floating microtubules, whereas pretreated cells exposed to Paclitaxel in the presence of Nocodazole-assembled centrosome organize microtubules. Nocodazole disrupts microtubules by binding to β-tubulin. Nocodazole prevents the formation of one of the two interchain disulfide linkages. Nocodazole impairs the transport of vesicles. Nocodazole suppress METH-induced cell death and lysosomal dysfunction. METH-induced cell death is significantly decreased by Nocodazole pretreatment in comparison to METH alone. Nocodazole doubles HDR efficiency to up to 30% in iPSCs. It improves the CRISPR-mediated HDR efficiency and has an additive effect on enhancing precise genome editing.
In vivo
The tumor volume and tumor weight of the mice treated with Ketoconazole \u2009plus\u2009 Nocodazole are significantly reduced as compared with those treated with Ketoconazole or Nocodazole alone. Combined treatment with Ketoconazole \u2009plus\u2009 Nocodazole strongly enhances apoptosis of COLO 205 tumor xenografts treated with Ketoconazole \u2009or\u2009 Nocodazole alone.
Cell Data
cell lines：
Concentrations：
Incubation Time：
Powder Purity:≥99%
| Isomeric SMILES | COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3 |
|---|---|
| WGK Germany | 3 |
| RTECS | DD6521000 |
| UN Number | 2811 |
| Packing Group | I |
| Molecular Weight | 301.32 |
| Beilstein | 1085978 |
| Reaxy-Rn | 1018009 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1018009&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 F2425005 | Certificate of Analysis | May 11, 2026 | N408505 |
| Solubility | Solubility (25°C) In vitro DMSO: 47 mg/mL (198.85 mM); Ethanol: 47 mg/mL (198.85 mM); Water: Insoluble; |
|---|---|
| Melt Point(°C) | 260 °C |
| 1. Li Qiwei, Chen Zaozao, Zhang Ying, Ding Shuang, Ding Haibo, Wang Luping, Xie Zhuoying, Fu Yifu, Wei Mengxiao, Liu Shengnan, Chen Jialun, Wang Xuan, Gu Zhongze. (2023) Imaging cellular forces with photonic crystals. Nature Communications, 14 (1): (1-14). [PMID:37963911] [10.1038/s41467-023-43090-9] |
| 2. Yuan-Yuan Liu, Yong-Yue Sun, Yuan Guo, Lu-Lu Chen, Jun-Hao Guo, Haifang Wang. (2025) Surface Charge Affects the Intracellular Fate and Clearance Dynamics of CdSe/ZnS Quantum Dots in Macrophages. Nanomaterials, 15 (15): (1189). [PMID:40801727] [10.3390/nano15151189] |
| 3. Yuan-Yuan Liu, Jie Liu, Hao Wu, Qiangqiang Zhang, Xue-Rui Tang, Dan Li, Chen-Si Li, Yuanfang Liu, Aoneng Cao, Haifang Wang. (2022) Endocytosis, Distribution, and Exocytosis of Polystyrene Nanoparticles in Human Lung Cells. Nanomaterials, 13 (1): (84). [PMID:36615994] [10.3390/nano13010084] |
| 4. Junzhao Yuan, Xiaoqian Huang, Yumeng Zhao, Jianhong Gu, Yan Yuan, Zongping Liu, Hui Zou, Jianchun Bian. (2022) Rat Hepatocytes Mitigate Cadmium Toxicity by Forming Annular Gap Junctions and Degrading Them via Endosome–Lysosome Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (24): (15607). [PMID:36555247] [10.3390/ijms232415607] |
| 5. Siqin Chen, Quan Zhou, Guowei Wang, Zhuxian Zhou, Jianbin Tang, Tao Xie, Youqing Shen. (2021) Effect of Cationic Charge Density on Transcytosis of Polyethylenimine. BIOMACROMOLECULES, [PMID:34775750] [10.1021/acs.biomac.1c01109] |
| 6. Wenjie Feng, Wanrui Shi, Shuwei Liu, Huiwen Liu, Yi Liu, Pengfei Ge, Hao Zhang. (2021) Fe(III)-Shikonin Supramolecular Nanomedicine for Combined Therapy of Tumor via Ferroptosis and Necroptosis. Advanced Healthcare Materials, 11 (2): (2101926). [PMID:34738742] [10.1002/adhm.202101926] |
| 7. Chen Xiaolong, Hu Cuihua, Zhang Yanan, Hao Wenjing, He Xu, Li Qing, Huang Yuxi, Huang Yibing, Chen Yuxin. (2020) Anticancer Activity and Mechanism of Action of kla-TAT Peptide. International Journal of Peptide Research and Therapeutics, 26 (4): (2285-2296). [PMID:] [10.1007/s10989-020-10019-5] |
| 8. Peng Zhang, Bangbang Li, Jianwei Du, Youxiang Wang. (2017) Regulation the morphology of cationized gold nanoparticles for effective gene delivery. COLLOIDS AND SURFACES B-BIOINTERFACES, [PMID:28558339] [10.1016/j.colsurfb.2017.04.056] |
| 9. Mengxin Zhang, Yingyu Zhang, Qionglin Wang, Kangbo Liu, Lifeng Li, Zhidan Yu, Ligong Hou, Xianwei Zhang, Huan Zhao, Weyland Cheng, Wancun Zhang. (2024) An APE1 gated signal amplified biosensor driven by catalytic hairpin assembly for the specific imaging of microRNA in situ. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:38307426] [10.1016/j.ijbiomac.2024.129902] |
| 10. Mengxin Zhang, Yingyu Zhang, Xianwei Zhang, Kangbo Liu, Lifeng Li, Zhidan Yu, Jingya Yuan, Wancun Zhang. (2024) An Enzymatically Activated and Catalytic Hairpin Assembly-Driven Intelligent AND-Gated DNA Network for Tumor Molecular Imaging. ANALYTICAL CHEMISTRY, [PMID:38836421] [10.1021/acs.analchem.4c02076] |
| 11. Mengxin Zhang, Yingyu Zhang, Xianwei Zhang, Qionglin Wang, Kangbo Liu, Muchun Yu, Lifeng Li, Zhidan Yu, Huan Zhao, Weyland Cheng, Wancun Zhang. (2024) An enzymatically activated self-powered and self-recycled strategy for in situ tumor cell-specific molecular imaging. CHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.cej.2024.151226] |
| 12. Dan Li, Gaoyang Yi, Guifang Cao, Adam C. Midgley, Yongli Yang, Dan Yang, Wenguang Liu, Yujuan He, Xiaolin Yao, Guoliang Li. (2025) Dual-Carriers of Tartary Buckwheat-Derived Exosome-Like Nanovesicles Synergistically Regulate Glucose Metabolism in the Intestine-Liver Axis. Small, [PMID:40079102] [10.1002/smll.202410124] |
| 13. Xingkang Diao, GuoHua Qi, Xinli Li, Yu Tian, Jing Li, Yongdong Jin. (2025) Label-Free Exosomal SERS Detection Assisted by Machine Learning for Accurately Discriminating Cell Cycle Stages and Revealing the Molecular Mechanisms during the Mitotic Process. ANALYTICAL CHEMISTRY, [PMID:39999424] [10.1021/acs.analchem.4c06240] |
| 14. Yue Wang, Ping Wang, Haiyan Li, Xiaoran Han, Haibin Zhu, Xiangqun Jin. (2024) Nanocrystal-Loaded Lipid Carriers for Improved Oral Absorption and Anticancer Efficacy of Etoposide: Formulation Development, Transport Mechanism, In Vitro and In Vivo Evaluation. MOLECULAR PHARMACEUTICS, [PMID:38329909] [10.1021/acs.molpharmaceut.3c00785] |
| 15. Yang Chen, Wenzhe Xu, Hao Jin, Mengsi Zhang, Shuwei Liu, Yi Liu, Hao Zhang. (2024) Nutritional Glutamine-Modified Iron-Delivery System with Enhanced Endocytosis for Ferroptosis Therapy of Pancreatic Tumors. ACS Nano, [PMID:39512234] [10.1021/acsnano.4c08083] |
| 16. Dan Yang, Yuqi Feng, Ying Yuan, Linxuan Zhang, Yao Zhou, Adam C. Midgley, Yanrong Wang, Ning Liu, Guoliang Li, Xiaolin Yao, Dechun Liu. (2024) Protein Coronas Derived from Mucus Act as Both Spear and Shield to Regulate Transferrin Functionalized Nanoparticle Transcellular Transport in Enterocytes. ACS Nano, [PMID:38417159] [10.1021/acsnano.3c11315] |
| 17. Huan Tang, Jie Zhou, Tong Yang, Hai-Ning Lyu, Zheng Chu, Ying Zhang, Ang Ma, Junzhe Zhang, Yuqing Meng, Chong Qiu, Sheng-Tao Yang, Jigang Wang, Zipeng Gong. (2025) Understanding the biological identity of metal-organic framework through profiling proteomic fingerprinting of protein corona. CHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.cej.2025.161320] |
| 18. Minghui Hu, Yingyu Zhang, Jie Zhang, Xianwei Zhang, Mengxin Zhang, Kangbo Liu, Lifeng Li, Zhidan Yu, Fei Zhang, Wancun Zhang. (2025) Double Self-Amplified Programmable Allosteric DNA Nanomachine for Enzymatically Triggered Spatially Controllable Molecular Imaging. ADVANCED FUNCTIONAL MATERIALS, [PMID:] [10.1002/adfm.202501940] |
| 19. Yuan-Yuan Liu, Jie Liu, Yuan Guo, Qiangqiang Zhang, Aoneng Cao, Haifang Wang. (2025) Interactions between polystyrene nanoparticles and human intestinal epithelial Caco-2 cells. NanoImpact, [PMID:40220994] [10.1016/j.impact.2025.100559] |
| 20. Xinchen Zhao, Liyan Qiu. (2025) Alkaline phosphatase-responsive lipid-like polyphosphonitrile nanovesicles to enhance immunotherapy and inhibit metastasis of triple-negative breast cancer. CHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.cej.2025.163576] |
| 21. Wancun Zhang, Zhenzhen Tian, Pin Zhang, Lifeng Li, Zhidan Yu, Meng Sun, Kangbo Liu, Ligong Hou, Jie Zhang, Mengxin Zhang, Xianwei Zhang. (2025) In vivo molecular imaging of drug resistance in neuroblastoma using APE1 cyclically cleaved and miR-514a self-recycled programmable allosteric DNA nanomachine. CHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.cej.2025.164581] |
| 22. Zheng Liu-Ting, Yan Zeng-Shuai, Li Xin-Yue, Chang Jia-Jia, Tan Xiao-Qi, Wang Yu-Xing, Ding Hong-Ming, Liu Qin, Ma Yu-Qiang, Huo Da. (2025) Nanomaterial signatures program biomolecular condensates via triphasic separation for chemoplasticity remodeling. Nature Communications, 16 (1): (1-24). [PMID:41162367] [10.1038/s41467-025-64623-4] |
| 23. Jingbo Sun, Liushunhao Chen, Hongchen Li, Chen Xu, Dong Xu, Jiali Ren. (2025) Green-emissive carbon dots for multitarget staining of cells, fungi, and bacteria: Insights into mechanisms and clinical applications. TALANTA, [PMID:40749512] [10.1016/j.talanta.2025.128625] |
| 24. Jingwen Xie, Jingxing Si, Xiaobei Deng, Jing Tan, Ruijie Zhu, Jizhou Qi, Gaoxia Ma, Bin Wang, Yi Wang, Yunzhou Guo, Yuping Chen, Shiqun Shao, Youqing Shen. (2026) Overcoming intranasal delivery barriers with ultrastable polyzwitterionic siRNA nanocages for enhanced glioblastoma therapy. BMEMat, [PMID:] [10.1002/bmm2.70068] |
